Universität zu Köln

Przyklenk, Matthias, Heumueller, Stefanie Elisabeth, Freiburg, Carolin, Luetke, Steffen, Sengle, Gerhard, Koch, Manuel ORCID: 0000-0002-2962-7814, Paulsson, Mats, Schiavinato, Alvise and Wagener, Raimund (2022). Lack of evidence for a role of anthrax toxin receptors as surface receptors for collagen VI and for its cleaved-off C5 domain/endotrophin. iScience, 25 (10). CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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Abstract

The microfibril-forming collagen VI is proteolytically cleaved and it was proposed that the released C-terminal Kunitz domain (C5) of the alpha 3 chain is an adipokine important for tumor progression and fibrosis. Designated endotrophin, C5 is a potent biomarker for fibroinflammatory diseases. However, the biochemical mechanisms behind endotrophin activity were not investigated. Earlier, anthrax toxin receptor 1 was found to bind C5, but this potential interaction was not further studied. Given the proposed physiological role of endotrophin, we aimed to determine how the signal is transmitted. Surprisingly, we could not detect any interaction between endotrophin and anthrax toxin receptor 1 or its close relative, anthrax toxin receptor 2. Moreover, we detect no binding of fully assembled collagen VI to either receptor. We also studied the collagen VI receptor NG2 (CSPG4) and confirmed that NG2 binds assembled collagen VI, but not cleaved C5/endotrophin. A cellular receptor for C5/endotrophin, therefore, still remains elusive.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Przyklenk, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Heumueller, Stefanie Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Freiburg, Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Luetke, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sengle, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiavinato, Alvise UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-663663
DOI:	10.1016/j.isci.2022.105116
Journal or Publication Title:	iScience
Volume:	25
Number:	10
Date:	2022
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2589-0042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHONDROITIN SULFATE PROTEOGLYCAN; INFANTILE SYSTEMIC HYALINOSIS; CAPILLARY MORPHOGENESIS; ALPHA-3(VI) CHAIN; EXTRACELLULAR-MATRIX; CELLULAR RECEPTOR; NG2 PROTEOGLYCAN; DOMAIN; EXPRESSION; MUTATIONS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66366