Universität zu Köln

Wanney, Johannes, Luesebrink, Jessica, Spoelgen, Gina, Demuth, Sabrina, Schildgen, Verena and Schildgen, Oliver (2022). Direct comparison of Altona-SARS-CoV-2 dual target RT-qPCR Assay with commercial LAMP Assay using throat washes in health care staff testing. J. Clin. Virol. Plus, 2 (3). AMSTERDAM: ELSEVIER. ISSN 2667-0380

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Abstract

Background: Rapid molecular diagnostics by PCR has a crucial role in handling the global SARS-CoV-2 pandemic. As diagnoses are time-sensitive and global supply chains are susceptible to various factors alternative detection methods would be an important backup. Objectives: During the study the performance of a commercially available isothermal LAMP method for SARSCoV-2 detection was compared to a IVD RT-PCR Assays using throat wash specimens that were routinely taken in our hospital setting. Study design: Throat wash specimens of hospital staff (n = 174) previously tested positive for SARS-CoV-2 by the Altona Diagnostics RealStar SARS-CoV-2 RT-PCR (Altona Diagnostics, Hamburg, Germany) was tested for SARS-CoV-2 also by the SARS-CoV-2 Rapid Colorimetric LAMP Assay (NEB Germany GmbH, Frankfurt a.M., Germany). Results: The sensitivity of the colorimetric LAMP Assay compared to RT-qPCR was 78.74%, and the specificity was determined to 88.24% with a positive predictive value of 0.986 and a negative predicitve value of 0.882. The positive and negative likelihood ratio for LAMP was 6.693 and 0.241, respectively, while the diagnostic odds ratio was 27.77. Conclusions: In times of limited PCR test ressources and in settings with limited PCR capacities, the colorimetric LAMP Assay could serve as an alternative, if a calculable loss of sensitivity is acceptable from the Public Health perspective in certain settings.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wanney, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Luesebrink, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Spoelgen, Gina UNSPECIFIED UNSPECIFIED UNSPECIFIED Demuth, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-663782
DOI:	10.1016/j.jcvp.2022.100088
Journal or Publication Title:	J. Clin. Virol. Plus
Volume:	2
Number:	3
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	2667-0380
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Infectious Diseases; Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66378