Universität zu Köln

Gu, Binbin, Goldfuss, Bernd and Dickschat, Jeroen S. S. (2023). Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens. Angew. Chem.-Int. Edit., 62 (1). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3773

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Abstract

The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme engineering through site-directed mutagenesis gave access to a high-yielding enzyme variant that provided six additional minor products and the main product in sufficient quantities to study its chemistry.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gu, Binbin UNSPECIFIED UNSPECIFIED UNSPECIFIED Goldfuss, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Dickschat, Jeroen S. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-664946
DOI:	10.1002/anie.202215688
Journal or Publication Title:	Angew. Chem.-Int. Edit.
Volume:	62
Number:	1
Date:	2023
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3773
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GERANYL PYROPHOSPHATE; BIOSYNTHESIS; CYCLIZATION; PRENYLTRANSFERASE; DIPHOSPHATE; ENZYMES Multiple languages Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66494