Universität zu Köln

Gao, He ORCID: 0000-0001-8001-5699, Song, Wen ORCID: 0000-0002-9498-2409, Severing, Edouard, Vayssieres, Alice, Huettel, Bruno, Franzen, Rainer, Richter, Rene, Chai, Jijie and Coupland, George ORCID: 0000-0001-6988-4172 (2021). PIF4 enhances DNA binding of CDF2 to co-regulate target gene expression and promote Arabidopsis hypocotyl cell elongation. Nat. Plants, 8 (9). S. 1082 - 1104. BERLIN: NATURE PORTFOLIO. ISSN 2055-0278

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Abstract

How specificity is conferred within gene regulatory networks is an important problem in biology. The basic helix-loop-helix PHYTOCHROME-INTERACTING FACTORs (PIFs) and single zinc-finger CYCLING DOF FACTORs (CDFs) mediate growth responses of Arabidopsis to light and temperature. We show that these two classes of transcription factor (TF) act cooperatively. CDF2 and PIF4 are temporally and spatially co-expressed, they interact to form a protein complex and act in the same genetic pathway to promote hypocotyl cell elongation. Furthermore, PIF4 substantially strengthens genome-wide occupancy of CDF2 at a subset of its target genes. One of these, YUCCA8, encodes an auxin biosynthesis enzyme whose transcription is increased by PIF4 and CDF2 to contribute to hypocotyl elongation. The binding sites of PIF4 and CDF2 in YUCCA8 are closely spaced, and in vitro PIF4 enhances binding of CDF2. We propose that this occurs by direct protein interaction and because PIF4 binding alters DNA conformation. Thus, we define mechanisms by which PIF and CDF TFs cooperate to achieve regulatory specificity and promote cell elongation in response to light. Hypocotyl cell elongation is promoted by combinatorial action of PIF4 and CDF2. This occurs because PIF4 enhances binding of CDF2 to common target genes increasing their transcription. These genes include the auxin biosynthesis gene YUCCA8.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gao, He UNSPECIFIED orcid.org/0000-0001-8001-5699 UNSPECIFIED Song, Wen UNSPECIFIED orcid.org/0000-0002-9498-2409 UNSPECIFIED Severing, Edouard UNSPECIFIED UNSPECIFIED UNSPECIFIED Vayssieres, Alice UNSPECIFIED UNSPECIFIED UNSPECIFIED Huettel, Bruno UNSPECIFIED UNSPECIFIED UNSPECIFIED Franzen, Rainer UNSPECIFIED UNSPECIFIED UNSPECIFIED Richter, Rene UNSPECIFIED UNSPECIFIED UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED Coupland, George UNSPECIFIED orcid.org/0000-0001-6988-4172 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-665289
DOI:	10.1038/s41477-022-01213-y
Journal or Publication Title:	Nat. Plants
Volume:	8
Number:	9
Page Range:	S. 1082 - 1104
Date:	2021
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2055-0278
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRANSCRIPTION FACTORS; CRYSTAL-STRUCTURE; PLANT-GROWTH; DOF DOMAIN; RECOGNITION; COINCIDENCE; DIVERSITY; INTERACTS; MECHANISM; REVEALS Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66528