Universität zu Köln

Brinkmann, Julia, Zeissler, Eva-Carina, Scharrenberg, Jan Simon, Schenk, Julia, Majjouti, Mohamed, Oberste, Max, Yagdiran, Ayla, Scheyerer, Max J., Jung, Norma, Siewe, Jan and Mahabir, Esther (2022). The diagnostic value of cytokines for the discrimination of vertebral osteomyelitis and degenerative diseases of the spine. Cytokine, 150. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1096-0023

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Abstract

Vertebral osteomyelitis (VO) is a primary infection of the endplates of the vertebral bodies with secondary infection of the adjacent intervertebral discs. Diagnosis is often delayed due to unspecific symptoms and a lack of specific infection markers. In this prospective study, we determined the suitability of 27 cytokines for the discrimination of VO and degenerative diseases of the spine and compared its diagnostic potential in relation to the C-reactive protein (CRP), which is widely used as a non-specific inflammation marker in clinical diagnostics. The patients included in this study underwent surgical stabilization of the lumbar and/or thoracic spine with removal of 1 or more affected intervertebral discs, as therapy for VO (n = 16) or for erosive osteochondrosis (EO, control group, n = 20). We evaluated the cytokine and CRP concentrations before (pre-OP = -20-0d where 0 means the day of surgery) and after surgery (post-OP) on days 3-5, 6-11, 40-56, and 63-142. Compared to the control patients pre-OP, a significantly higher elevation of the 4 cytokines IL-6, IL-8, IL-12 (p70), and VEGF as well as CRP were found in the VO patients, showing an area under the curve > 0.80 pre-OP. No significant differences were observed between VO patients with high and low virulent bacteria with respect to all 5 elevated biomarkers. This is the first prospective study in which a broad spectrum of 27 cytokines was analysed via multiplex assay using sera from patients with and without VO. Our results show that, in addition to CRP, 4 different cytokines were significantly altered in VO but not control patients. The results implicate that these candidate cytokines may be used in a multiplex assay for discrimination between VO and degenerative diseases of the spine.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brinkmann, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeissler, Eva-Carina UNSPECIFIED UNSPECIFIED UNSPECIFIED Scharrenberg, Jan Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Schenk, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Majjouti, Mohamed UNSPECIFIED UNSPECIFIED UNSPECIFIED Oberste, Max UNSPECIFIED UNSPECIFIED UNSPECIFIED Yagdiran, Ayla UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheyerer, Max J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jung, Norma UNSPECIFIED UNSPECIFIED UNSPECIFIED Siewe, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahabir, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-665836
DOI:	10.1016/j.cyto.2021.155782
Journal or Publication Title:	Cytokine
Volume:	150
Date:	2022
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1096-0023
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language C-REACTIVE PROTEIN; LOW-BACK-PAIN; PROINFLAMMATORY CYTOKINE; ANTIBIOTIC-THERAPY; CLINICAL-FEATURES; MODIC CHANGES; GROWTH-FACTOR; SPONDYLODISCITIS; INFECTION; INTERLEUKIN-8 Multiple languages Biochemistry & Molecular Biology; Cell Biology; Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66583