Universität zu Köln

Katsinas, Nikolaos, Gehlsen, Uta ORCID: 0000-0001-9057-9199, Garcia-Posadas, Laura ORCID: 0000-0001-9921-8541, Rodriguez-Rojo, Soraya ORCID: 0000-0002-9054-1462, Steven, Philipp ORCID: 0000-0001-6892-3619, Gonzalez-Garcia, Maria J. and Enriquez-de-Salamanca, Amalia (2022). Olive Pomace Phenolic Compounds: From an Agro-Industrial By-Product to a Promising Ocular Surface Protection for Dry Eye Disease. J. Clin. Med., 11 (16). BASEL: MDPI. ISSN 2077-0383

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Abstract

Dry eye (DED) is a prevalent disease with immune-mediated inflammation as the principal pathophysiological etiology. Olive pomace, the major by-product of the olive oil industry, is rich in high-value polyphenols. Their anti-inflammatory and immunomodulatory activities were determined on human CD4+ T cells (hTCD4+) and in a DED animal model. The viability of hTCD4+ cells isolated from peripheral blood and activated with phytohemagglutinin-M was evaluated after treatment for 48 h with an olive pomace extract (OPT3, 0.10-0.40 mg/mL) and its major compound, hydroxytyrosol (25-100 mu M). Regarding the DED animal model, 100 mu M hydroxytyrosol, 0.20 mg/mL OPT3, or vehicle (borate buffer) were topically administered to 14 days-desiccating stress-exposed (constant airflow/scopolamine administration) C57BL/6 mice. Tear volume, corneal fluorescein staining (CFS), CD4+, and CD8+ T cell count in lymph nodes (flow cytometry), and IP-10 and TNF-alpha gene expression (qRT-PCR) in the cornea, conjunctiva, and lacrimal glands were evaluated. OPT3 (0.2-0.4 mg/mL) and hydroxytyrosol (100 mu M) significantly reduced hTCD4+ proliferation. In mice, both treatments reduced lacrimal gland IP-10 gene expression. OPT3 also decreased CFS, and conjunctival IP-10 and corneal TNF-alpha gene expression. In lymph nodes, hydroxytyrosol reduced CD3+, OPT3, and CD8+ count. Thus, a high-value application as a promising DED protection was proposed for olive pomace.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Katsinas, Nikolaos UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehlsen, Uta UNSPECIFIED orcid.org/0000-0001-9057-9199 UNSPECIFIED Garcia-Posadas, Laura UNSPECIFIED orcid.org/0000-0001-9921-8541 UNSPECIFIED Rodriguez-Rojo, Soraya UNSPECIFIED orcid.org/0000-0002-9054-1462 UNSPECIFIED Steven, Philipp UNSPECIFIED orcid.org/0000-0001-6892-3619 UNSPECIFIED Gonzalez-Garcia, Maria J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Enriquez-de-Salamanca, Amalia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-666174
DOI:	10.3390/jcm11164703
Journal or Publication Title:	J. Clin. Med.
Volume:	11
Number:	16
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-0383
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPIGALLOCATECHIN GALLATE; MOUSE MODEL; INFLAMMATORY CYTOKINES; THERAPEUTIC-EFFICACY; CYCLOSPORINE-A; MILL WASTES; HYDROXYTYROSOL; OIL; ACTIVATION; CORNEAL Multiple languages Medicine, General & Internal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66617