Universität zu Köln

Meyer, Christina, Lucassen, Kai, Gerson, Stefanie, Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Wille, Thorsten, Seifert, Harald ORCID: 0000-0002-7732-4762 and Higgins, Paul G. (2022). Contribution of RND-Type Efflux Pumps in Reduced Susceptibility to Biocides in Acinetobacter baumannii. Antibiotics-Basel, 11 (11). BASEL: MDPI. ISSN 2079-6382

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Abstract

Bacterial efflux pumps are among the key mechanisms of resistance against antibiotics and biocides. We investigated whether differential expression levels of the RND-type efflux pumps AdeABC and AdeIJK impacted the susceptibility to commonly used biocides in multidrug-resistant Acinetobacter baumannii. Susceptibility testing and time-kill assays of defined laboratory and clinical A. baumannii strains with different levels of efflux pump expression were performed after exposure to the biocides benzalkonium chloride, chlorhexidine digluconate, ethanol, glucoprotamin, octenidine dihydrochloride, and triclosan. While the impact of efflux pump expression on susceptibility to the biocides was limited, noticeable differences were found in kill curves, where AdeABC expression correlated with greater survival after exposure to benzalkonium chloride, chlorhexidine digluconate, glucoprotamin, and octenidine dihydrochloride. AdeABC expression levels did not impact kill kinetics with ethanol nor triclosan. In conclusion, these data indicate that the overexpression of the RND-type efflux pumps AdeABC and AdeIJK contributes to the survival of A. baumannii when exposed to residual concentrations of biocides.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Meyer, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Lucassen, Kai UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerson, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED orcid.org/0000-0001-8591-8184 UNSPECIFIED Wille, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED orcid.org/0000-0002-7732-4762 UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-666373
DOI:	10.3390/antibiotics11111635
Journal or Publication Title:	Antibiotics-Basel
Volume:	11
Number:	11
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2079-6382
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PSEUDOMONAS-AERUGINOSA; TRICLOSAN RESISTANCE; MULTIDRUG-RESISTANCE; EXPRESSION; DISINFECTANTS; ANTIBIOTICS; ADEIJK; FAMILY; MUTANT; ADEABC Multiple languages Infectious Diseases; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66637