Universität zu Köln

Marciniak, Pawel ORCID: 0000-0002-4790-001X, Pacholska-Bogalska, Joanna and Ragionieri, Lapo ORCID: 0000-0003-0099-2719 (2022). Neuropeptidomes of Tenebrio molitor L. and Zophobas atratus Fab. (Coleoptera, Polyphaga: Tenebrionidae). J. Proteome Res., 21 (10). S. 2247 - 2261. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

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Abstract

Neuropeptides are signaling molecules that regulate almost all physiological processes in animals. Around 50 different genes for neuropeptides have been described in insects. In Coleoptera, which is the largest insect order based on numbers of described species, knowledge about neuropeptides and protein hormones is still limited to a few species. Here, we analyze the neuropeptidomes of two closely related tenebrionid beetles: Tenebrio molitor and Zophobas atratus-both of which are model species in physiological and pharmacological research. We combined transcriptomic and mass spectrometry analyses of the central nervous system to identify neuropeptides and neuropeptide-like and protein hormones. Several precursors were identified in T. molitor and Z. atratus, of which 50 and 40, respectively, were confirmed by mass spectrometry. This study provides the basis for further functional studies of neuropeptides as well as for the design of environmentally friendly and species specific peptidomimetics to be used as biopesticides. Furthermore, since T. molitor has become accepted by the European Food Safety Authority as a novel food, a deeper knowledge of the neuropeptidome of this species will prove useful for optimizing production programs at an industrial scale.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Marciniak, Pawel UNSPECIFIED orcid.org/0000-0002-4790-001X UNSPECIFIED Pacholska-Bogalska, Joanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Ragionieri, Lapo UNSPECIFIED orcid.org/0000-0003-0099-2719 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-668268
DOI:	10.1021/acs.jproteome.1c00694
Journal or Publication Title:	J. Proteome Res.
Volume:	21
Number:	10
Page Range:	S. 2247 - 2261
Date:	2022
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RED FLOUR BEETLE; MASS-SPECTROMETRY; DROSOPHILA-MELANOGASTER; FUNCTIONAL EXPRESSION; TRIBOLIUM-CASTANEUM; MOLECULAR-CLONING; PEPTIDE-HORMONES; NERVOUS-SYSTEM; INSECT; IDENTIFICATION Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66826