Universität zu Köln

Goertemaker, Katharina, Yee, Chad, Bartoelke, Rabea, Behrmann, Heide, Voss, Jan-Oliver ORCID: 0000-0002-1817-9200, Schmidt, Jessica ORCID: 0000-0002-8393-4532, Xu, Jingjing, Solovyeva, Vita, Leberecht, Bo ORCID: 0000-0001-9672-1504, Behrmann, Elmar ORCID: 0000-0001-6794-3669, Mouritsen, Henrik ORCID: 0000-0001-7082-4839 and Koch, Karl-Wilhelm ORCID: 0000-0003-1501-0044 (2022). Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein. Cells, 11 (13). BASEL: MDPI. ISSN 2073-4409

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Abstract

Background: Night-migratory birds sense the Earth's magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the alpha-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein-protein interaction studies include surface plasmon resonance, pulldown affinity binding and Forster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Forster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goertemaker, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Yee, Chad UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartoelke, Rabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Behrmann, Heide UNSPECIFIED UNSPECIFIED UNSPECIFIED Voss, Jan-Oliver UNSPECIFIED orcid.org/0000-0002-1817-9200 UNSPECIFIED Schmidt, Jessica UNSPECIFIED orcid.org/0000-0002-8393-4532 UNSPECIFIED Xu, Jingjing UNSPECIFIED UNSPECIFIED UNSPECIFIED Solovyeva, Vita UNSPECIFIED UNSPECIFIED UNSPECIFIED Leberecht, Bo UNSPECIFIED orcid.org/0000-0001-9672-1504 UNSPECIFIED Behrmann, Elmar UNSPECIFIED orcid.org/0000-0001-6794-3669 UNSPECIFIED Mouritsen, Henrik UNSPECIFIED orcid.org/0000-0001-7082-4839 UNSPECIFIED Koch, Karl-Wilhelm UNSPECIFIED orcid.org/0000-0003-1501-0044 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-668444
DOI:	10.3390/cells11132043
Journal or Publication Title:	Cells
Volume:	11
Number:	13
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SURFACE-PLASMON RESONANCE; ALPHA-SUBUNIT; GUANYLATE-CYCLASE; STRUCTURAL-CHANGE; MAGNETIC-FIELD; BINDING SITES; TRANSDUCIN; MECHANISM; MAGNETORECEPTION; ACTIVATION Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66844