Universität zu Köln

Shobo, Adeola ORCID: 0000-0002-2846-323X, Roentgen, Alexander, Hancock, Mark A. and Multhaup, Gerhard ORCID: 0000-0003-1373-0048 (2022). Biophysical characterization as a tool to predict amyloidogenic and toxic properties of amyloid-beta 42 peptides. FEBS Lett., 596 (11). S. 1401 - 1412. HOBOKEN: WILEY. ISSN 1873-3468

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Abstract

Amyloid-beta 42 (A beta 42) peptides are central to the amyloid pathology in Alzheimer's disease (AD). As biological mimetics, properties of synthetic A beta peptides usually vary between vendors and batches, thus impacting the reproducibility of experimental studies. Here, we tested recombinantly expressed A beta 42 (Aspl to A1a42) against synthetic A beta 42 from different suppliers using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), circular dichroism (CD) spectroscopy, thioflavin T aggregation, surface plasmon resonance, and MTT cell viability assays. Overall, our recombinant A beta 42 provided a reproducible mimetic of desired properties. Across experimental approaches, the combined detection of A beta 42 dimers and random coil to beta-sheet transition only correlated with aggregation-prone and cytotoxic peptides. Conclusively, combining MALDI-MS with CD appears to provide a rapid, reliable means to predict the 'bioactivity' of A beta 42.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shobo, Adeola UNSPECIFIED orcid.org/0000-0002-2846-323X UNSPECIFIED Roentgen, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Hancock, Mark A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Multhaup, Gerhard UNSPECIFIED orcid.org/0000-0003-1373-0048 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-670648
DOI:	10.1002/1873-3468.14358
Journal or Publication Title:	FEBS Lett.
Volume:	596
Number:	11
Page Range:	S. 1401 - 1412
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1873-3468
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language A-BETA OLIGOMER; ALZHEIMERS-DISEASE; PROTEIN; BRAIN; FIBRILLOGENESIS; PURIFICATION; EXPRESSION; HYPOTHESIS; PRECURSOR; FIBRILS Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67064