Universität zu Köln

Kowatschew, Daniel, Nia, Shahrzad Bozorg, Hassan, Shahzaib, Ustinova, Jana, Weth, Franco and Korsching, Sigrun, I (2022). Spatial organization of olfactory receptor gene choice in the complete V1R-related ORA family of zebrafish. Sci Rep, 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

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Abstract

The vertebrate sense of smell employs four main receptor families for detection of odors, among them the V1R/ORA family, which is unusually small and highly conserved in teleost fish. Zebrafish possess just seven ORA receptors, enabling a comprehensive analysis of the expression patterns of the entire family. The olfactory organ of zebrafish is representative for teleosts, cup-shaped, with lamella covered with sensory epithelium protruding into the cup from a median raphe. We have performed quantitative in situ hybridization on complete series of horizontal cryostat sections of adult zebrafish olfactory organ, and have analysed the location of ora-expressing cells in three dimensions, radial diameter, laminar height, and height-within-the-organ. We report broadly overlapping, but distinctly different distributions for all ora genes, even for ora3a and ora3b, the most recent gene duplication. Preferred positions in different dimensions are independent of each other. This spatial logic is very similar to previous reports for the much larger families of odorant receptor (or) and V2R-related olfC genes in zebrafish. Preferred positions for ora genes tend to be more central and more apical than those we observed for these other two families, consistent with expression in non-canonical sensory neuron types.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kowatschew, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Nia, Shahrzad Bozorg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hassan, Shahzaib UNSPECIFIED UNSPECIFIED UNSPECIFIED Ustinova, Jana UNSPECIFIED UNSPECIFIED UNSPECIFIED Weth, Franco UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun, I UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-671049
DOI:	10.1038/s41598-022-17900-x
Journal or Publication Title:	Sci Rep
Volume:	12
Number:	1
Date:	2022
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ZONAL ORGANIZATION; EXPRESSION DOMAINS; SENSORY NEURONS; CRYPT NEURONS; EPITHELIUM; SEGREGATION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67104