Terminal H-reflex Measurements in Mice - Kölner UniversitätsPublikationsServer

Wieters, Frederique, Gruhn, Matthias, Bueschges, Ansgar, Fink, Gereon R. ORCID: 0000-0002-8230-1856 and Aswendt, Markus (2022). Terminal H-reflex Measurements in Mice. J. Vis. Exp. (184). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

Full text not available from this repository.

DOI:10.3791/63304

Abstract

The Hoffmann reflex (H-reflex), as an electrical analog to the stretch reflex, allows electrophysiological validation of the integrity of neural circuits after injuries such as spinal cord damage or stroke. An increase of the H-reflex response, together with symptoms like non-voluntary muscle contractions, pathologically augmented stretch reflex, and hypertonia in the corresponding muscle, is an indicator of post-stroke spasticity (PSS). In contrast to rather nerve-unspecific transcutaneous measurements, here, we present a protocol to quantify the H-reflex directly at the ulnar and median nerves of the forepaw, which is applicable, with minor modifications, to the tibial and sciatic nerve of the hindpaw. Based on the direct stimulation and the adaptation to different nerves, the method represents a reliable and versatile tool to validate electrophysiological changes in spasticity-related disease models.

Item Type:

Journal Article

Creators:

Creators	Email	ORCID	ORCID Put Code
Wieters, Frederique	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Gruhn, Matthias	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Bueschges, Ansgar	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Fink, Gereon R.	UNSPECIFIED	orcid.org/0000-0002-8230-1856	UNSPECIFIED
Aswendt, Markus	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED