Universität zu Köln

Wu, Ming-Hua, Huang, Lu-Yao, Sun, Li-Xiao, Qian, Hui, Wei, Yun-Yun, Liang, Shuang, Zhu, Xue-Ming, Li, Lin, Lu, Jian-Ping, Lin, Fu-Cheng and Liu, Xiao-Hong (2022). A Putative D-Arabinono-1,4-lactone Oxidase, MoAlo1, Is Required for Fungal Growth, Conidiogenesis, and Pathogenicity in Magnaporthe oryzae. J. Fungi, 8 (1). BASEL: MDPI. ISSN 2309-608X

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Abstract

Magnaporthe oryzae is the causal agent of rice blast outbreaks. L-ascorbic acid (ASC) is a famous antioxidant found in nature. However, while ASC is rare or absent in fungi, a five-carbon analog, D-erythroascorbic acid (EASC), seems to appear to be a substitute for ASC. Although the antioxidant function of ASC has been widely described, the specific properties and physiological functions of EASC remain poorly understood. In this study, we identified a D-arabinono-1,4-lactone oxidase (ALO) domain-containing protein, MoAlo1, and found that MoAlo1 was localized to mitochondria. Disruption of MoALO1 (Delta Moalo1) exhibited defects in vegetative growth as well as conidiogenesis. The Delta Moalo1 mutant was found to be more sensitive to exogenous H2O2. Additionally, the pathogenicity of conidia in the Delta Moalo1 null mutant was reduced deeply in rice, and defective penetration of appressorium-like structures (ALS) formed by the hyphal tips was also observed in the Delta Moalo1 null mutant. When exogenous EASC was added to the conidial suspension, the defective pathogenicity of the Delta Moalo1 mutant was restored. Collectively, MoAlo1 is essential for growth, conidiogenesis, and pathogenicity in M. oryzae.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wu, Ming-Hua UNSPECIFIED UNSPECIFIED UNSPECIFIED Huang, Lu-Yao UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Li-Xiao UNSPECIFIED UNSPECIFIED UNSPECIFIED Qian, Hui UNSPECIFIED UNSPECIFIED UNSPECIFIED Wei, Yun-Yun UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, Shuang UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhu, Xue-Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Lin UNSPECIFIED UNSPECIFIED UNSPECIFIED Lu, Jian-Ping UNSPECIFIED UNSPECIFIED UNSPECIFIED Lin, Fu-Cheng UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Xiao-Hong UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-671913
DOI:	10.3390/jof8010072
Journal or Publication Title:	J. Fungi
Volume:	8
Number:	1
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language D-ERYTHROASCORBIC ACID; VITAMIN-C BIOSYNTHESIS; ASCORBIC-ACID; PENETRATION; ENZYME; DIFFERENTIATION; PURIFICATION; AUTOPHAGY; MOLECULE; PLANTS Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67191