Universität zu Köln

Jakobsen, Jens Rithamer, Mackey, Abigail Louise, Koch, Manuel ORCID: 0000-0002-2962-7814, Imhof, Thomas, Hannibal, Jens, Kjaer, Michael and Krogsgaard, Michael Rindom (2023). Larger interface area at the human myotendinous junction in type 1 compared with type 2 muscle fibers. Scand. J. Med. Sci. Sports, 33 (2). S. 136 - 146. HOBOKEN: WILEY. ISSN 1600-0838

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Abstract

The myotendinous junction (MTJ) is structurally specialized to transmit force. The highly folded muscle membrane at the MTJ increases the contact area between muscle and tendon and potentially the load tolerance of the MTJ. Muscles with a high content of type II fibers are more often subject to strain injury compared with muscles with type I fibers. It is hypothesized that this is explained by a smaller interface area of MTJ in type II compared with type I muscle fibers. The aim was to investigate by confocal microscopy whether there is difference in the surface area at the MTJ between type I and II muscle fibers. Individual muscle fibers with an intact MTJ were isolated by microscopic dissection in samples from human semitendinosus, and they were labeled with antibodies against collagen XXII (indicating MTJ) and type I myosin (MHCI). Using a spinning disc confocal microscope, the MTJ from each fiber was scanned and subsequently reconstructed to a 3D-model. The interface area between muscle and tendon was calculated in type I and II fibers from these reconstructions. The MTJ was analyzed in 314 muscle fibers. Type I muscle fibers had a 22% larger MTJ interface area compared with type II fibers (p < 0.05), also when the area was normalized to fiber diameter. By the new method, it was possible to analyze the structure of the MTJ from a large number of human muscle fibers. The finding that the interface area between muscle and tendon is higher in type I compared with type II fibers suggests that type II fibers are less resistant to strain and therefore more susceptible to injury.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jakobsen, Jens Rithamer UNSPECIFIED UNSPECIFIED UNSPECIFIED Mackey, Abigail Louise UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Imhof, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hannibal, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Kjaer, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Krogsgaard, Michael Rindom UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-673359
DOI:	10.1111/sms.14246
Journal or Publication Title:	Scand. J. Med. Sci. Sports
Volume:	33
Number:	2
Page Range:	S. 136 - 146
Date:	2023
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-0838
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NORDIC HAMSTRING EXERCISE; FORCE TRANSMISSION; STEREOLOGICAL ANALYSIS; TENDON JUNCTIONS; SOCCER PLAYERS; SLOW FIBERS; INJURIES; FOOTBALL; EPIDEMIOLOGY; PREVENTION Multiple languages Sport Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67335