Bindics, Janos ORCID: 0000-0001-8778-3439, Khan, Mamoona ORCID: 0000-0003-3301-9536, Uhse, Simon, Kogelmann, Benjamin ORCID: 0000-0003-4796-6315, Baggely, Laura, Reumann, Daniel ORCID: 0000-0002-4594-8212, Ingole, Kishor D., Stirnberg, Alexandra, Rybecky, Anna, Darino, Martin ORCID: 0000-0003-0190-5314, Navarrete, Fernando, Doehlemann, Gunther and Djamei, Armin ORCID: 0000-0002-8087-9566 (2022). Many ways to TOPLESS - manipulation of plant auxin signalling by a cluster of fungal effectors. New Phytol., 236 (4). S. 1455 - 1471. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Plant biotrophic pathogens employ secreted molecules, called effectors, to suppress the host immune system and redirect the host's metabolism and development in their favour. Putative effectors of the gall-inducing maize pathogenic fungus Ustilago maydis were analysed for their ability to induce auxin signalling in plants. Using genetic, biochemical, cell-biological, and bioinformatic approaches we functionally elucidate a set of five, genetically linked effectors, called Topless (TPL) interacting protein (Tips) effectors that induce auxin signalling. We show that Tips induce auxin signalling by interfering with central corepressors of the TPL family. CRISPR-Cas9 mutants and deletion strain analysis indicate that the auxin signalling inducing subcluster effectors plays a redundant role in virulence. Although none of the Tips seem to have a conserved interaction motif, four of them bind solely to the N-terminal TPL domain and, for Tip1 and Tip4, we demonstrate direct competition with auxin/indole-3-acetic acid transcriptional repressors for their binding to TPL class of corepressors. Our findings reveal that TPL proteins, key regulators of growth-defence antagonism, are a major target of the U. maydis effectome.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Bindics, JanosUNSPECIFIEDorcid.org/0000-0001-8778-3439UNSPECIFIED
Khan, MamoonaUNSPECIFIEDorcid.org/0000-0003-3301-9536UNSPECIFIED
Uhse, SimonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kogelmann, BenjaminUNSPECIFIEDorcid.org/0000-0003-4796-6315UNSPECIFIED
Baggely, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reumann, DanielUNSPECIFIEDorcid.org/0000-0002-4594-8212UNSPECIFIED
Ingole, Kishor D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stirnberg, AlexandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rybecky, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Darino, MartinUNSPECIFIEDorcid.org/0000-0003-0190-5314UNSPECIFIED
Navarrete, FernandoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doehlemann, GuntherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Djamei, ArminUNSPECIFIEDorcid.org/0000-0002-8087-9566UNSPECIFIED
URN: urn:nbn:de:hbz:38-677524
DOI: 10.1111/nph.18315
Journal or Publication Title: New Phytol.
Volume: 236
Number: 4
Page Range: S. 1455 - 1471
Date: 2022
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1469-8137
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
USTILAGO-MAYDIS; TRANSCRIPTIONAL REPRESSORS; SMUT FUNGI; ARABIDOPSIS; PROTEINS; EVOLUTION; INTERACTOME; EXPRESSION; RESISTANCE; MECHANISMMultiple languages
Plant SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/67752

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