Universität zu Köln

Perez-Anton, Miguel ORCID: 0000-0003-4071-7340, Schneider, Ilsa ORCID: 0000-0001-5065-8757, Kroll, Patrizia ORCID: 0000-0002-7308-2292, Hofhuis, Hugo, Metzger, Sabine, Pauly, Markus ORCID: 0000-0002-3116-2198 and Hay, Angela (2022). Explosive seed dispersal depends on SPL7 to ensure sufficient copper for localized lignin deposition via laccases. Proc. Natl. Acad. Sci. U. S. A., 119 (24). WASHINGTON: NATL ACAD SCIENCES. ISSN 1091-6490

Full text not available from this repository.

Abstract

Exploding seed pods evolved in the Arabidopsis relative Cardamine hirsuta via morphomechanical innovations that allow the storage and rapid release of elastic energy. Asymmetric lignin deposition within endocarpb cell walls is one such innovation that is required for explosive seed dispersal and evolved in association with the trait. However, the genetic control of this novel lignin pattern is unknown. Here, we identify three lignin-polymerizing laccases, LAC4, 11, and 17, that precisely colocalize with, and are redundantly required for, asymmetric lignification of endocarpb cells. By screening for C. hirsuta mutants with less lignified fruit valves, we found that loss of function of the transcription factor gene SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 7 (SPL7) caused a reduction in endocarpb cell-wall lignification and a consequent reduction in seed dispersal range. SPL7 is a conserved regulator of copper homeostasis and is both necessary and sufficient for copper to accumulate in the fruit. Laccases are copperrequiring enzymes. We discovered that laccase activity in endocarpb cell walls depends on the SPL7 pathway to acclimate to copper deficiency and provide sufficient copper for lignin polymerization. Hence, SPL7 links mineral nutrition to efficient dispersal of the next generation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Perez-Anton, Miguel UNSPECIFIED orcid.org/0000-0003-4071-7340 UNSPECIFIED Schneider, Ilsa UNSPECIFIED orcid.org/0000-0001-5065-8757 UNSPECIFIED Kroll, Patrizia UNSPECIFIED orcid.org/0000-0002-7308-2292 UNSPECIFIED Hofhuis, Hugo UNSPECIFIED UNSPECIFIED UNSPECIFIED Metzger, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Pauly, Markus UNSPECIFIED orcid.org/0000-0002-3116-2198 UNSPECIFIED Hay, Angela UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-678023
DOI:	10.1073/pnas.2202287119
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	119
Number:	24
Date:	2022
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	1091-6490
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; GENE FAMILY; SBP-DOMAIN; LIGNIFICATION; PEROXIDASES; POLYMERIZATION; CHLAMYDOMONAS; TRANSCRIPTION; HOMEOSTASIS; EXPRESSION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67802