Ercanoglu, Meryem Seda, Gieselmann, Lutz ORCID: 0000-0003-3699-3318, Daehling, Sabrina, Poopalasingam, Nareshkumar ORCID: 0000-0002-4981-6209, Detmer, Susanne, Koch, Manuel ORCID: 0000-0002-2962-7814, Korenkov, Michael ORCID: 0000-0002-2988-8483, Halwe, Sandro, Kluever, Michael, Di Cristanziano, Veronica, Janicki, Hanna, Schlotz, Maike, Worczinski, Johanna, Gathof, Birgit, Gruell, Henning, Zehner, Matthias ORCID: 0000-0001-5745-0836, Becker, Stephan, Vanshylla, Kanika ORCID: 0000-0003-4552-9170, Kreer, Christoph ORCID: 0000-0002-9140-9850 and Klein, Florian ORCID: 0000-0003-1376-1792 (2022). No substantial preexisting B cell immunity against SARS-CoV-2 in healthy adults. iScience, 25 (3). CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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Abstract

Preexisting immunity against SARS-CoV-2 may have critical implications for our understanding of COVID-19 susceptibility and severity. The presence and clinical relevance of a preexisting B cell immunity remain to be fully elucidated. Here, we provide a detailed analysis of the B cell immunity to SARS-CoV-2 in unexposed individuals. To this end, we extensively investigated SARS-CoV-2 humoral immunity in 150 adults sampled pre-pandemically. Comprehensive screening of donor plasma and purified IgG sampler, for bin Jing and neutralization in various functional assays revealed no substantial activity against SARS-CoV-2 but broad reactivity to endemic betacoronaviruses. Moreover, we analyzed antibody sequences of 8,174 putatively SARS-CoV-2-reactive B cells at a single cell level and generated and tested 158 monoclonal antibodies. None of these antibodies displayed relevant binding or neutralizing activity against SARS-CoV-2. Taken together, our results show no evidence of competent preexisting antibody and B cell immunity against SARS-CoV-2 in unexposed adults.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ercanoglu, Meryem SedaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gieselmann, LutzUNSPECIFIEDorcid.org/0000-0003-3699-3318UNSPECIFIED
Daehling, SabrinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Poopalasingam, NareshkumarUNSPECIFIEDorcid.org/0000-0002-4981-6209UNSPECIFIED
Detmer, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, ManuelUNSPECIFIEDorcid.org/0000-0002-2962-7814UNSPECIFIED
Korenkov, MichaelUNSPECIFIEDorcid.org/0000-0002-2988-8483UNSPECIFIED
Halwe, SandroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kluever, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Cristanziano, VeronicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Janicki, HannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schlotz, MaikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Worczinski, JohannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gathof, BirgitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gruell, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zehner, MatthiasUNSPECIFIEDorcid.org/0000-0001-5745-0836UNSPECIFIED
Becker, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vanshylla, KanikaUNSPECIFIEDorcid.org/0000-0003-4552-9170UNSPECIFIED
Kreer, ChristophUNSPECIFIEDorcid.org/0000-0002-9140-9850UNSPECIFIED
Klein, FlorianUNSPECIFIEDorcid.org/0000-0003-1376-1792UNSPECIFIED
URN: urn:nbn:de:hbz:38-678389
DOI: 10.1016/j.isci.2022.103951
Journal or Publication Title: iScience
Volume: 25
Number: 3
Date: 2022
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 2589-0042
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ORIGINAL ANTIGENIC SIN; POTENT NEUTRALIZING ANTIBODIES; HUMAN CORONAVIRUS; DENGUE VIRUS; INFECTION; DISEASE; GENERATION; CHILDREN; SEQUENCEMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/67838

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