Kakanj, Parisa, Bhide, Sourabh, Moussian, Bernard and Leptin, Maria ORCID: 0000-0001-7097-348X (2022). Autophagy-mediated plasma membrane removal promotes the formation of epithelial syncytia. Embo J., 41 (12). HOBOKEN: WILEY. ISSN 1460-2075

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Abstract

Epithelial wound healing in Drosophila involves the formation of multinucleate cells surrounding the wound. We show that autophagy, a cellular degradation process often deployed in stress responses, is required for the formation of a multinucleated syncytium during wound healing, and that autophagosomes that appear near the wound edge acquire plasma membrane markers. In addition, uncontrolled autophagy in the unwounded epidermis leads to the degradation of endo-membranes and the lateral plasma membrane, while apical and basal membranes and epithelial barrier function remain intact. Proper functioning of TORC1 is needed to prevent destruction of the larval epidermis by autophagy, in a process that depends on phagophore initiation and expansion but does not require autophagosomes fusion with lysosomes. Autophagy induction can also affect other sub-cellular membranes, as shown by its suppression of experimentally induced laminopathy-like nuclear defects. Our findings reveal a function for TORC1-mediated regulation of autophagy in maintaining membrane integrity and homeostasis in the epidermis and during wound healing.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kakanj, ParisaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bhide, SourabhUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moussian, BernardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leptin, MariaUNSPECIFIEDorcid.org/0000-0001-7097-348XUNSPECIFIED
URN: urn:nbn:de:hbz:38-678664
DOI: 10.15252/embj.2021109992
Journal or Publication Title: Embo J.
Volume: 41
Number: 12
Date: 2022
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1460-2075
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NUCLEAR PROTEINS KUGELKERN; CELL-GROWTH; DROSOPHILA; LAMIN; INDUCTION; POLYPLOIDIZATION; OVEREXPRESSION; REGENERATION; DYSFUNCTION; RECRUITMENTMultiple languages
Biochemistry & Molecular Biology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/67866

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