Universität zu Köln

Hasan, M. Shamim, Chopra, Divykriti, Damm, Anika, Koprivova, Anna, Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Meyer, Andreas J., Mueller-Schuessele, Stefanie, Grundler, Florian M. W. and Siddique, Shahid ORCID: 0000-0001-7503-4318 (2022). Glutathione contributes to plant defence against parasitic cyst nematodes. Mol. Plant Pathol., 23 (7). S. 1048 - 1060. HOBOKEN: WILEY. ISSN 1364-3703

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Abstract

Cyst nematodes (CNs) are an important group of root-infecting sedentary endoparasites that severely damage many crop plants worldwide. An infective CN juvenile enters the host's roots and migrates towards the vascular cylinder, where it induces the formation of syncytial feeding cells, which nourish the CN throughout its parasitic stages. Here, we examined the role of glutathione (l-gamma-glutamyl-l-cysteinyl-glycine) in Arabidopsis thaliana on infection with the CN Heterodera schachtii. Arabidopsis lines with mutations pad2, cad2, or zir1 in the glutamate-cysteine ligase (GSH1) gene, which encodes the first enzyme in the glutathione biosynthetic pathway, displayed enhanced CN susceptibility, but susceptibility was reduced for rax1, another GSH1 allele. Biochemical analysis revealed differentially altered thiol levels in these mutants that was independent of nematode infection. All glutathione-deficient mutants exhibited impaired activation of defence marker genes as well as genes for biosynthesis of the antimicrobial compound camalexin early in infection. Further analysis revealed a link between glutathione-mediated plant resistance to CN infection and the production of camalexin on nematode infection. These results suggest that glutathione levels affect plant resistance to CN by fine-tuning the balance between the cellular redox environment and the production of compounds related to defence against infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hasan, M. Shamim UNSPECIFIED UNSPECIFIED UNSPECIFIED Chopra, Divykriti UNSPECIFIED UNSPECIFIED UNSPECIFIED Damm, Anika UNSPECIFIED UNSPECIFIED UNSPECIFIED Koprivova, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Meyer, Andreas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Schuessele, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Grundler, Florian M. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Siddique, Shahid UNSPECIFIED orcid.org/0000-0001-7503-4318 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-678997
DOI:	10.1111/mpp.13210
Journal or Publication Title:	Mol. Plant Pathol.
Volume:	23
Number:	7
Page Range:	S. 1048 - 1060
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1364-3703
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; HETERODERA-SCHACHTII; GENE-EXPRESSION; CELL-DIVISION; HOST; BIOSYNTHESIS; STRESS; DEFICIENT; ASCORBATE; HOMEOSTASIS Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67899