Universität zu Köln

Wohlfarth, Esther ORCID: 0000-0001-9756-7385, Kresken, Michael ORCID: 0000-0003-0613-925X, Higgins, Paul G., Stefanik, Danuta, Wille, Julia, Hafner, Dieter, Koerber-Irrgang, Barbara and Seifert, Harald ORCID: 0000-0002-7732-4762 (2022). The evolution of carbapenem resistance determinants and major epidemiological lineages among carbapenem-resistant Acinetobacter baumannii isolates in Germany, 2010-2019. Int. J. Antimicrob. Agents, 60 (5-6). AMSTERDAM: ELSEVIER. ISSN 1872-7913

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Abstract

The aim of this study was to investigate and compare the molecular epidemiology and carbapenem resis-tance determinants in clinical Acinetobacter baumannii isolates collected during four multicentre surveil-lance studies conducted by the Paul-Ehrlich-Society for Infection Therapy. Isolates were collected prospec-tively from hospital in-patients at 17 medical centres in Germany over four periods of three-to six -months starting in October of each of 2010, 2013, 2016 and 2019. Species identification was performed by MALDI-TOF, gyrB multiplex polymerase chain reaction (PCR), and detection of the intrinsic blaOXA-51-like gene. Minimum inhibitory concentrations were determined by broth microdilution. The prevalence of carbapenemase-encoding genes was investigated by OXA-multiplex PCR and whole-genome sequencing. Molecular epidemiology was examined by rep-PCR and core-genome multi-locus sequence typing. A to-tal of 302 A. baumannii isolates were collected. Resistance to imipenem and/or meropenem was detected in 58 isolates (19.2%) from 14 centres. The proportion of carbapenem-resistant isolates increased from 21.3% in 2010 to 33.3% in 2013, and then decreased to 13.8% in 2016 and 12.3% in 2019. Forty-six of these isolates were associated with the international clonal lineage IC2 and five with IC1. The most prevalent carbapenemase gene detected was blaOXA-23-like (n = 51). Further carbapenem-resistance determinants were blaOXA-40-like (n = 1), blaOXA-58-like (n = 3) and blaNDM-1 (n = 2). In one isolate, ISAba1 was detected upstream of blaOXA-51-like. In conclusion, IC2 was the most prevalent clonal lineage detected in this study. Interestingly, in Germany, carbapenem resistance seems to have decreased in A. baumannii between 2013 and 2019.(c) 2022 Published by Elsevier Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wohlfarth, Esther UNSPECIFIED orcid.org/0000-0001-9756-7385 UNSPECIFIED Kresken, Michael UNSPECIFIED orcid.org/0000-0003-0613-925X UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stefanik, Danuta UNSPECIFIED UNSPECIFIED UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Hafner, Dieter UNSPECIFIED UNSPECIFIED UNSPECIFIED Koerber-Irrgang, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED orcid.org/0000-0002-7732-4762 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-679168
DOI:	10.1016/j.ijantimicag.2022.106689
Journal or Publication Title:	Int. J. Antimicrob. Agents
Volume:	60
Number:	5-6
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1872-7913
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67916