Universität zu Köln

Macabrey, Diane, Joniova, Jaroslava, Gasser, Quentin, Bechelli, Clemence, Longchamp, Alban, Urfer, Severine, Lambelet, Martine, Fu, Chun-Yu, Schwarz, Guenter, Wagnieres, Georges, Deglise, Sebastien and Allagnat, Florent ORCID: 0000-0001-6528-679X (2022). Sodium thiosulfate, a source of hydrogen sulfide, stimulates endothelial cell proliferation and neovascularization. Front. Cardiovasc. Med., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2297-055X

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Abstract

Therapies to accelerate vascular repair are currently lacking. Pre-clinical studies suggest that hydrogen sulfide (H2S), an endogenous gasotransmitter, promotes angiogenesis. Here, we hypothesized that sodium thiosulfate (STS), a clinically relevant source of H2S, would stimulate angiogenesis and vascular repair. STS stimulated neovascularization in WT and LDLR receptor knockout mice following hindlimb ischemia as evidenced by increased leg perfusion assessed by laser Doppler imaging, and capillary density in the gastrocnemius muscle. STS also promoted VEGF-dependent angiogenesis in matrigel plugs in vivo and in the chorioallantoic membrane of chick embryos. In vitro, STS and NaHS stimulated human umbilical vein endothelial cell (HUVEC) migration and proliferation. Seahorse experiments further revealed that STS inhibited mitochondrial respiration and promoted glycolysis in HUVEC. The effect of STS on migration and proliferation was glycolysis-dependent. STS probably acts through metabolic reprogramming of endothelial cells toward a more proliferative glycolytic state. These findings may hold broad clinical implications for patients suffering from vascular occlusive diseases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Macabrey, Diane UNSPECIFIED UNSPECIFIED UNSPECIFIED Joniova, Jaroslava UNSPECIFIED UNSPECIFIED UNSPECIFIED Gasser, Quentin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bechelli, Clemence UNSPECIFIED UNSPECIFIED UNSPECIFIED Longchamp, Alban UNSPECIFIED UNSPECIFIED UNSPECIFIED Urfer, Severine UNSPECIFIED UNSPECIFIED UNSPECIFIED Lambelet, Martine UNSPECIFIED UNSPECIFIED UNSPECIFIED Fu, Chun-Yu UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagnieres, Georges UNSPECIFIED UNSPECIFIED UNSPECIFIED Deglise, Sebastien UNSPECIFIED UNSPECIFIED UNSPECIFIED Allagnat, Florent UNSPECIFIED orcid.org/0000-0001-6528-679X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-679464
DOI:	10.3389/fcvm.2022.965965
Journal or Publication Title:	Front. Cardiovasc. Med.
Volume:	9
Date:	2022
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	2297-055X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ANGIOGENESIS; ARTERIOGENESIS; H2S; PREVALENCE; DISEASE; VEGF Multiple languages Cardiac & Cardiovascular Systems Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67946