Steuernagel, Lukas ORCID: 0000-0003-0043-1436, Lam, Brian Y. H., Klemm, Paul ORCID: 0000-0002-5985-1737, Dowsett, Georgina K. C., Bauder, Corinna A., Tadross, John A., Hitschfeld, Tamara Sotelo, Martin, Almudena del Rio, Chen, Weiyi, de Solis, Alain J., Fenselau, Henning, Davidsen, Peter, Cimino, Irene, Kohnke, Sara N., Rimmington, Debra, Coll, Anthony P., Beyer, Andreas, Yeo, Giles S. H. and Bruening, Jens C. (2022). HypoMap-a unified single-cell gene expression atlas of the murine hypothalamus. Nat. Metab., 4 (10). S. 1402 - 1434. BERLIN: NATURE PORTFOLIO. ISSN 2522-5812

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Abstract

The hypothalamus plays a key role in coordinating fundamental body functions. Despite recent progress in single-cell technologies, a unified catalog and molecular characterization of the heterogeneous cell types and, specifically, neuronal subtypes in this brain region are still lacking. Here, we present an integrated reference atlas, 'HypoMap,' of the murine hypothalamus, consisting of 384,925 cells, with the ability to incorporate new additional experiments. We validate HypoMap by comparing data collected from Smart-Seq+Fluidigm C1 and bulk RNA sequencing of selected neuronal cell types with different degrees of cellular heterogeneity. Finally, via HypoMap, we identify classes of neurons expressing glucagon-like peptide-1 receptor (Glp1r) and prepronociceptin (Pnoc), and validate them using single-molecule in situ hybridization. Collectively, HypoMap provides a unified framework for the systematic functional annotation of murine hypothalamic cell types, and it can serve as an important platform to unravel the functional organization of hypothalamic neurocircuits and to identify druggable targets for treating metabolic disorders.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Steuernagel, LukasUNSPECIFIEDorcid.org/0000-0003-0043-1436UNSPECIFIED
Lam, Brian Y. H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klemm, PaulUNSPECIFIEDorcid.org/0000-0002-5985-1737UNSPECIFIED
Dowsett, Georgina K. C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauder, Corinna A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tadross, John A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hitschfeld, Tamara SoteloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Martin, Almudena del RioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chen, WeiyiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
de Solis, Alain J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fenselau, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Davidsen, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cimino, IreneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kohnke, Sara N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rimmington, DebraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Coll, Anthony P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beyer, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yeo, Giles S. H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bruening, Jens C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-681820
DOI: 10.1038/s42255-022-00657-y
Journal or Publication Title: Nat. Metab.
Volume: 4
Number: 10
Page Range: S. 1402 - 1434
Date: 2022
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2522-5812
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INTEGRATED ANALYSIS; ENERGY-BALANCE; POPULATIONS; RECEPTOR; ORGANIZATION; HOMEOSTASIS; GLIOSIS; OBESITYMultiple languages
Endocrinology & MetabolismMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68182

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