Universität zu Köln

Huang, Shijia, Jia, Aolin, Song, Wen ORCID: 0000-0002-9498-2409, Hessler, Giuliana ORCID: 0000-0002-5923-5571, Meng, Yonggang, Sun, Yue, Xu, Lina, Laessle, Henriette, Jirschitzka, Jan ORCID: 0000-0003-1007-4700, Ma, Shoucai, Xiao, Yu, Yu, Dongli, Hou, Jiao, Liu, Ruiqi, Sun, Huanhuan, Liu, Xiaohui, Han, Zhifu, Chang, Junbiao, Parker, Jane E. and Chai, Jijie (2022). Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity. Science, 377 (6605). S. 487 - 495. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/ interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) activity for immune signaling. TIR-NLR signaling requires the helper NLRs N requirement gene 1 (NRG1), Activated Disease Resistance 1 (ADR1), and Enhanced Disease Susceptibility 1 (EDS1), which forms a heterodimer with each of its paralogs Phytoalexin Deficient 4 (PAD4) and Senescence-Associated Gene 101 (SAG101). Here, we show that TIR-containing proteins catalyze the production of 2'-(5''-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP) in vitro and in planta. Biochemical and structural data demonstrate that EDS1PAD4 is a receptor complex for pRib-AMP and pRib-ADP, which allosterically promote EDS1-PAD4 interaction with ADR1-L1 but not NRG1A. Our study identifies TIR-catalyzed pRib-AMP and pRib-ADP as a missing link in TIR signaling through EDS1-PAD4 and as likely second messengers for plant immunity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huang, Shijia UNSPECIFIED UNSPECIFIED UNSPECIFIED Jia, Aolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Song, Wen UNSPECIFIED orcid.org/0000-0002-9498-2409 UNSPECIFIED Hessler, Giuliana UNSPECIFIED orcid.org/0000-0002-5923-5571 UNSPECIFIED Meng, Yonggang UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Lina UNSPECIFIED UNSPECIFIED UNSPECIFIED Laessle, Henriette UNSPECIFIED UNSPECIFIED UNSPECIFIED Jirschitzka, Jan UNSPECIFIED orcid.org/0000-0003-1007-4700 UNSPECIFIED Ma, Shoucai UNSPECIFIED UNSPECIFIED UNSPECIFIED Xiao, Yu UNSPECIFIED UNSPECIFIED UNSPECIFIED Yu, Dongli UNSPECIFIED UNSPECIFIED UNSPECIFIED Hou, Jiao UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Ruiqi UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Huanhuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Xiaohui UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Zhifu UNSPECIFIED UNSPECIFIED UNSPECIFIED Chang, Junbiao UNSPECIFIED UNSPECIFIED UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-682389
DOI:	10.1126/science.abq3297
Journal or Publication Title:	Science
Volume:	377
Number:	6605
Page Range:	S. 487 - 495
Date:	2022
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	1095-9203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-DEATH; PATHOGEN EFFECTOR; SIGNALING DOMAIN; RESISTOSOME; PROTEINS; COMPLEX Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68238