Universität zu Köln

Koser, Franziska, Hobbach, Anastasia J., Abdellatif, Mahmoud, Herbst, Viktoria, Tuerk, Clara, Reinecke, Holger, Krueger, Marcus, Sedej, Simon and Linke, Wolfgang A. (2022). Acetylation and phosphorylation changes to cardiac proteins in experimental HFpEF due to metabolic risk reveal targets for treatment. Life Sci., 309. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1879-0631

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Abstract

Aims: Despite the high prevalence of heart failure with preserved ejection fraction (HFpEF), the pathomechan-isms remain elusive and specific therapy is lacking. Disease-causing factors include metabolic risk, notably obesity. However, proteomic changes in HFpEF are poorly understood, hampering therapeutic strategies. We sought to elucidate how metabolic syndrome affects cardiac protein expression, phosphorylation and acetylation in the Zucker diabetic fatty/Spontaneously hypertensive heart failure F1 (ZSF1) rat HFpEF model, and to evaluate changes regarding their potential for treatment.Main methods: ZSF1 obese and lean rats were fed a Purina diet up to the onset of HFpEF in the obese animals. We quantified the proteome, phosphoproteome and acetylome of ZSF1 obese versus lean heart tissues by mass spectrometry and singled out targets for site-specific evaluation.Key findings: The acetylome of ZSF1 obese versus lean hearts was more severely altered (21 % of proteins changed) than the phosphoproteome (9 %) or proteome (3 %). Proteomic alterations, confirmed by immuno-blotting, indicated low-grade systemic inflammation and endothelial remodeling in obese hearts, but low nitric oxide-dependent oxidative/nitrosative stress. Altered acetylation in ZSF1 obese hearts mainly affected pathways important for metabolism, energy production and mechanical function, including hypo-acetylation of mechan-ical proteins but hyper-acetylation of proteins regulating fatty acid metabolism. Hypo-acetylation and hypo-phosphorylation of elastic titin in ZSF1 obese hearts could explain myocardial stiffening.Significance: Cardiometabolic syndrome alters posttranslational modifications, notably acetylation, in experi-mental HFpEF. Pathway changes implicate a HFpEF signature of low-grade inflammation, endothelial dysfunction, metabolic and mechanical impairment, and suggest titin stiffness and mitochondrial metabolism as promising therapeutic targets.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koser, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Hobbach, Anastasia J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Abdellatif, Mahmoud UNSPECIFIED UNSPECIFIED UNSPECIFIED Herbst, Viktoria UNSPECIFIED UNSPECIFIED UNSPECIFIED Tuerk, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Reinecke, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Sedej, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Linke, Wolfgang A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-682877
DOI:	10.1016/j.lfs.2022.120998
Journal or Publication Title:	Life Sci.
Volume:	309
Date:	2022
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1879-0631
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PRESERVED EJECTION FRACTION; HEART-FAILURE; TITIN; INFLAMMATION; STIFFNESS; PARADIGM Multiple languages Medicine, Research & Experimental; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68287