Universität zu Köln

Wunderlich, Alexander ORCID: 0000-0002-8587-7631, Xanthopoulou, Kyriaki, Wille, Julia, Wohlfarth, Esther, Gerson, Stefanie, Kaase, Martin, Seifert, Harald and Higgins, Paul G. (2023). Carbapenem resistance in Acinetobacter pittii isolates mediated by metallo-beta-lactamases. J. Antimicrob. Chemother., 78 (2). S. 488 - 497. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2091

Full text not available from this repository.

Abstract

Objectives To characterize the genetic environment of metallo-beta-lactamases (MBL) in carbapenem-resistant clinical Acinetobacter pittii isolates. Methods Seventeen carbapenem-resistant A. pittii isolates harbouring an MBL were collected between 2010 and 2015 in Germany. Antimicrobial susceptibility testing was performed using agar dilution. Presence of MBLs was confirmed by PCR and their genetic location determined by S1-pulsed-field gel electrophoresis followed by Southern blot hybridization. Whole-genome sequencing was performed using the Miseq and MinION platforms. Isolates were typed using an ad hoc core genome MLST scheme. Conjugation into A. baumannii was tested by broth mating. Results In 10 isolates the MBL was plasmid-encoded and in seven isolates chromosomally encoded. bla(GIM-1) and bla(VIM-2) were plasmid-encoded, bla(VIM-4) was chromosomally encoded, while bla(NDM-1) was chromosomally encoded in four and plasmid-encoded in three isolates. Seven of ten plasmids were conjugative into A. baumannii. Although most isolates were unrelated, the backbones of the MBL-encoding plasmid showed >99% similarity and only differed in the MBL-encoding area. bla(NDM-1)-harbouring plasmids were highly similar to other plasmids from Acinetobacter isolates worldwide while the bla(VIM-2)- and bla(GIM-1)-encoding plasmids have not been described. Conclusions These data show the existence of a promiscuous plasmid circulating in A. pittii isolates in Germany that differs only in the MBL-encoding region. Its plasmid backbone has been found globally among multiple Acinetobacter spp. These data should raise awareness of an epidemic conjugative plasmid that has independently acquired MBLs. We should also consider that future comparative plasmid analysis will look beyond solely the resistome and include the mobile elements carrying the resistance genes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wunderlich, Alexander UNSPECIFIED orcid.org/0000-0002-8587-7631 UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED UNSPECIFIED UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlfarth, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerson, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaase, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-683061
DOI:	10.1093/jac/dkac418
Journal or Publication Title:	J. Antimicrob. Chemother.
Volume:	78
Number:	2
Page Range:	S. 488 - 497
Date:	2023
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2091
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOLECULAR CHARACTERIZATION; ANTIBIOTIC-RESISTANCE; SP NOV.; BAUMANNII; GENE; EMERGENCE; CALCOACETICUS; IDENTIFICATION; DIJKSHOORNIAE; PSEUDOMONAS Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68306