Universität zu Köln

Conrad, Thomas ORCID: 0000-0001-5618-6295 and Altmueller, Janine (2022). Single cell- and spatial 'Omics revolutionize physiology. Acta Physiol., 235 (4). HOBOKEN: WILEY. ISSN 1748-1716

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Abstract

Single cell multi- 'Omics and Spatial Transcriptomics are prominent technological highlights of recent years, and both fields still witness a ceaseless firework of novel approaches for high resolution profiling of additional omics layers. As all life processes in organs and organisms are based on the functions of their fundamental building blocks, the individual cells and their interactions, these methods are of utmost worth for the study of physiology in health and disease. Recent discoveries on embryonic development, tumor immunology, the detailed cellular composition and function of complex tissues like for example the kidney or the brain, different roles of the same cell type in different organs, the oncogenic program of individual tumor entities, or the architecture of immunopathology in infected tissue are based on single cell and spatial transcriptomics experiments. In this review, we will give a broad overview of technological concepts for single cell and spatial analysis, showing both advantages and limitations, and illustrate their impact with some particularly impressive case studies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Conrad, Thomas UNSPECIFIED orcid.org/0000-0001-5618-6295 UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-683219
DOI:	10.1111/apha.13848
Journal or Publication Title:	Acta Physiol.
Volume:	235
Number:	4
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1748-1716
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOME-WIDE EXPRESSION; IN-SITU HYBRIDIZATION; BRANCHED DNA BDNA; MESSENGER-RNA-SEQ; GENE-EXPRESSION; CHROMATIN STATES; TISSUE; ORGANIZATION; METHYLATION; PROFILES Multiple languages Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68321