Universität zu Köln

Wagner, Isabel Viola, Savchuk, Iuliia, Sahlin, Lena, Kulle, Alexandra, Kloeting, Nora, Dietrich, Arne, Holterhus, Paul-Martin, Doetsch, Joerg, Blueher, Matthias and Soeder, Olle (2022). De Novo and Depot-Specific Androgen Production in Human Adipose Tissue: A Source of Hyperandrogenism in Women with Obesity. Obes. Facts, 15 (2). S. 281 - 292. BASEL: KARGER. ISSN 1662-4033

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Abstract

Introduction: Obesity in women is often associated with hyperandrogenism, but the role of adipose tissue (AT) in androgen synthesis remains unclear.Therefore, we studied whether AT could be a source of androgens promoting hyperandrogenism. Methods: Subcutaneous and visceral (visc) AT was collected from lean and obese women. Androgen levels were evaluated in serum, AT, and cell-culture supernatant. Gene and protein expression of steroidogenic enzymes were determined. Results: Obese subjects had elevated serum androgen levels, which reduced after weight loss. Androgens were measurable in AT and in cell-culture supernatants of adipocytes. Steroids were higher in AT from obese women, with the highest difference for testosterone in visc AT (+7.9-fold, p = 0.032). Steroidogenic enzymes were expressed in human AT with depot-specific differences. Obese women showed a significantly higher expression of genes of the backdoor pathway and of CYP19 in visc AT. Conclusion: The whole steroidogenic machinery of the classical and backdoor pathways of steroidogenesis, and the capacity for androgen biosynthesis, were found in both AT depots and cultured adipocytes. Therefore, we hypothesize that AT is a de novo site of androgen production and the backdoor pathway of steroidogenesis might be a new pathomechanism for hyperandrogenism in women with obesity. (C) 2022 The Author(s). Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wagner, Isabel Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Savchuk, Iuliia UNSPECIFIED UNSPECIFIED UNSPECIFIED Sahlin, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Kulle, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloeting, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Dietrich, Arne UNSPECIFIED UNSPECIFIED UNSPECIFIED Holterhus, Paul-Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Blueher, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Soeder, Olle UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-683825
DOI:	10.1159/000521571
Journal or Publication Title:	Obes. Facts
Volume:	15
Number:	2
Page Range:	S. 281 - 292
Date:	2022
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1662-4033
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYCYSTIC-OVARY-SYNDROME; INSULIN-RESISTANCE; THECA CELLS; DEHYDROEPIANDROSTERONE-SULFATE; TESTOSTERONE; METABOLISM; EXPRESSION; FAT; STEROIDOGENESIS; PHENOTYPE Multiple languages Endocrinology & Metabolism; Nutrition & Dietetics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68382