Universität zu Köln

Ullah, Amin, Yu, Xiaojie, Odenthal, Margarete ORCID: 0000-0002-2424-0960, Meemboor, Sonja, Ahmad, Bashir, Rehman, Irshad ur, Ahmad, Jamshaid ORCID: 0000-0003-2105-1918, Ali, Qurban and Nadeem, Tariq ORCID: 0000-0002-0104-5484 (2022). Circulating microRNA-122 in HCV cirrhotic patients with high frequency of genotype 3. PLoS One, 17 (5). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

Full text not available from this repository.

Abstract

MicroRNA-122 (miR-122) is a liver abundant microRNA that is released upon liver injury. In the present study, we investigated the circulating miR-122 profiles in a Pakistani patients ' cohort with HCV chronic liver disease that was mainly based on HCV genotype 3 infections. From 222 patients with chronic HCV liver disease, classified as mild, moderate, or severe, serum samples were collected. Cell-free RNA was isolated and used for miR-122 quantification by qPCR. More than 60% of 222 patients were infected with HCV genotype 3. ALT values and HCV viral load showed no correlation with the HCV genotype. Circulating miR-122 levels were significantly upregulated in patients with cirrhosis. Notably, HCV patients with mild cirrhosis showed the most marked increase in serum miR-122 levels (p = 0.0001). Furthermore, we proved a positive correlation (r = 0.46) of miR-122 with the ALT values in patients with mild cirrhosis. Importantly, our data of increased miR-122 levels in serum samples obtained from a patient cohort with a high prevalence of chronic genotype 3 HCV infection confirmed the previous findings collected from cohorts with a high prevalence of genotype 1. Therefore, we suggest that miR-122 increase after HCV infection does not depend on the HCV genotype. In conclusion, our findings confirm that serum miR-122 levels are significantly upregulated in the HCV cirrhotic patients serving in particular as a biomarker for the non-advanced stages of cirrhosis, independently of the HCV genotype.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ullah, Amin UNSPECIFIED UNSPECIFIED UNSPECIFIED Yu, Xiaojie UNSPECIFIED UNSPECIFIED UNSPECIFIED Odenthal, Margarete UNSPECIFIED orcid.org/0000-0002-2424-0960 UNSPECIFIED Meemboor, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahmad, Bashir UNSPECIFIED UNSPECIFIED UNSPECIFIED Rehman, Irshad ur UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahmad, Jamshaid UNSPECIFIED orcid.org/0000-0003-2105-1918 UNSPECIFIED Ali, Qurban UNSPECIFIED UNSPECIFIED UNSPECIFIED Nadeem, Tariq UNSPECIFIED orcid.org/0000-0002-0104-5484 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-684351
DOI:	10.1371/journal.pone.0268526
Journal or Publication Title:	PLoS One
Volume:	17
Number:	5
Date:	2022
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEPATOCELLULAR-CARCINOMA; MIR-122; INFECTION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68435