Hansen, Jan N., Brueckner, Matthias, Pietrowski, Marie J., Jikeli, Jan F., Plescher, Monika, Beckert, Hannes, Schnaars, Mareike, Fuelle, Lorenz, Reitmeier, Katharina, Langmann, Thomas ORCID: 0000-0001-6826-529X, Foerster, Irmgard, Boche, Delphine ORCID: 0000-0002-5884-130X, Petzold, Gabor C. and Halle, Annett (2022). MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia. Mol. Biol. Cell, 33 (11). BETHESDA: AMER SOC CELL BIOLOGY. ISSN 1939-4586

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Abstract

Microglia are the primary resident innate immune cells of the CNS. They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become the gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy, and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Hansen, Jan N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brueckner, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pietrowski, Marie J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jikeli, Jan F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Plescher, MonikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beckert, HannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schnaars, MareikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fuelle, LorenzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reitmeier, KatharinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Langmann, ThomasUNSPECIFIEDorcid.org/0000-0001-6826-529XUNSPECIFIED
Foerster, IrmgardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boche, DelphineUNSPECIFIEDorcid.org/0000-0002-5884-130XUNSPECIFIED
Petzold, Gabor C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Halle, AnnettUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-685749
DOI: 10.1091/mbc.E21-11-0585
Journal or Publication Title: Mol. Biol. Cell
Volume: 33
Number: 11
Date: 2022
Publisher: AMER SOC CELL BIOLOGY
Place of Publication: BETHESDA
ISSN: 1939-4586
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MOUSE; BRAIN; ATP; RECONSTRUCTION; MACROPHAGES; RECEPTORS; PATHOLOGY; DYNAMICS; BEHAVIORMultiple languages
Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68574

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