Universität zu Köln

Hansen, Jan N., Brueckner, Matthias, Pietrowski, Marie J., Jikeli, Jan F., Plescher, Monika, Beckert, Hannes, Schnaars, Mareike, Fuelle, Lorenz, Reitmeier, Katharina, Langmann, Thomas ORCID: 0000-0001-6826-529X, Foerster, Irmgard, Boche, Delphine ORCID: 0000-0002-5884-130X, Petzold, Gabor C. and Halle, Annett (2022). MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia. Mol. Biol. Cell, 33 (11). BETHESDA: AMER SOC CELL BIOLOGY. ISSN 1939-4586

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Abstract

Microglia are the primary resident innate immune cells of the CNS. They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become the gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy, and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hansen, Jan N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brueckner, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Pietrowski, Marie J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jikeli, Jan F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plescher, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Beckert, Hannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnaars, Mareike UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuelle, Lorenz UNSPECIFIED UNSPECIFIED UNSPECIFIED Reitmeier, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Langmann, Thomas UNSPECIFIED orcid.org/0000-0001-6826-529X UNSPECIFIED Foerster, Irmgard UNSPECIFIED UNSPECIFIED UNSPECIFIED Boche, Delphine UNSPECIFIED orcid.org/0000-0002-5884-130X UNSPECIFIED Petzold, Gabor C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Halle, Annett UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-685749
DOI:	10.1091/mbc.E21-11-0585
Journal or Publication Title:	Mol. Biol. Cell
Volume:	33
Number:	11
Date:	2022
Publisher:	AMER SOC CELL BIOLOGY
Place of Publication:	BETHESDA
ISSN:	1939-4586
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOUSE; BRAIN; ATP; RECONSTRUCTION; MACROPHAGES; RECEPTORS; PATHOLOGY; DYNAMICS; BEHAVIOR Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68574