Universität zu Köln

Vogel, Emanuel ORCID: 0000-0001-6560-0136, Kocher, Katharina ORCID: 0000-0003-2331-3838, Priller, Alina ORCID: 0000-0002-4982-0347, Cheng, Cho-Chin ORCID: 0000-0002-5951-2743, Steininger, Philipp, Liao, Bo-Hung, Korber, Nina, Willmann, Annika, Irrgang, Pascal, Held, Juergen, Moosmann, Carolin, Schmidt, Viviane, Beileke, Stephanie, Wytopil, Monika, Heringer, Sarah, Bauer, Tanja, Brockhoff, Ronja, Jeske, Samuel, Mijocevic, Hrvoje, Christa, Catharina, Salmanton-Garcia, Jon, Tinnefeld, Kathrin, Bogdan, Christian, Yazici, Sarah ORCID: 0000-0003-1524-9071, Knolle, Percy, Cornely, Oliver A., Ueberla, Klaus, Protzer, Ulrike, Schober, Kilian and Tenbusch, Matthias (2022). Dynamics of humoral and cellular immune responses after homologous and heterologous SARS-CoV-2 vaccination with ChAdOx1 nCoV-19 and BNT162b2. EBioMedicine, 85. AMSTERDAM: ELSEVIER. ISSN 2352-3964

Full text not available from this repository.

Abstract

Background Vaccines are an important means to overcome the SARS-CoV-2 pandemic. They induce specific anti-body and T-cell responses but it remains open how well vaccine-induced immunity is preserved over time following homologous and heterologous immunization regimens. Here, we compared the dynamics of humoral and cellular immune responses up to 180 days after homologous or heterologous vaccination with either ChAdOx1-nCoV-19 (ChAd) or BNT162b2 (BNT) or both.Methods Various tests were used to determine the humoral and cellular immune response. To quantify the antibody levels, we used the surrogate neutralization (sVNT) assay from YHLO, which we augmented with pseudo-and real virus neutralization tests (pVNT and rVNT). Antibody avidity was measured by a modified ELISA. To determine cel-lular reactivity, we used an IFN-g Elispot, IFN-g/IL Flurospot, and intracellular cytokine staining.Findings Antibody responses significantly waned after vaccination, irrespective of the regimen. The capacity to neu-tralize SARS-CoV-2 -including variants of concern such as Delta or Omicron -was superior after heterologous compared to homologous BNT vaccination, both of which resulted in longer-lasting humoral immunity than homol-ogous ChAd immunization. All vaccination regimens induced stable, polyfunctional T-cell responses. Interpretation These findings demonstrate that heterologous vaccination with ChAd and BNT is a potent alternative to induce humoral and cellular immune protection in comparison to the homologous vaccination regimens.Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Vogel, Emanuel UNSPECIFIED orcid.org/0000-0001-6560-0136 UNSPECIFIED Kocher, Katharina UNSPECIFIED orcid.org/0000-0003-2331-3838 UNSPECIFIED Priller, Alina UNSPECIFIED orcid.org/0000-0002-4982-0347 UNSPECIFIED Cheng, Cho-Chin UNSPECIFIED orcid.org/0000-0002-5951-2743 UNSPECIFIED Steininger, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Liao, Bo-Hung UNSPECIFIED UNSPECIFIED UNSPECIFIED Korber, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Willmann, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Irrgang, Pascal UNSPECIFIED UNSPECIFIED UNSPECIFIED Held, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Moosmann, Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Viviane UNSPECIFIED UNSPECIFIED UNSPECIFIED Beileke, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Wytopil, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Heringer, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauer, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockhoff, Ronja UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeske, Samuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Mijocevic, Hrvoje UNSPECIFIED UNSPECIFIED UNSPECIFIED Christa, Catharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Salmanton-Garcia, Jon UNSPECIFIED UNSPECIFIED UNSPECIFIED Tinnefeld, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bogdan, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Yazici, Sarah UNSPECIFIED orcid.org/0000-0003-1524-9071 UNSPECIFIED Knolle, Percy UNSPECIFIED UNSPECIFIED UNSPECIFIED Cornely, Oliver A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ueberla, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Protzer, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Schober, Kilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Tenbusch, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-686353
DOI:	10.1016/j.ebiom.2022.104294
Journal or Publication Title:	EBioMedicine
Volume:	85
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	2352-3964
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEUTRALIZATION; MEMORY Multiple languages Medicine, General & Internal; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68635