Universität zu Köln

Hinkelbein, Jochen ORCID: 0000-0003-3585-9459, Johnson, Lydia Kolaparambil Varghese, Kiselev, Nikolai, Schmitz, Jan, Hellmich, Martin ORCID: 0000-0001-5174-928X, Drinhaus, Hendrik, Lichtenstein, Theresa, Storm, Christian and Adler, Christoph (2022). Proteomics-Based Serum Alterations of the Human Protein Expression after Out-of-Hospital Cardiac Arrest: Pilot Study for Prognostication of Survivors vs. Non-Survivors at Day 1 after Return of Spontaneous Circulation (ROSC). J. Clin. Med., 11 (4). BASEL: MDPI. ISSN 2077-0383

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Abstract

Background: Targeted temperature management (TTM) is considered standard therapy for patients after out-of-hospital cardiac arrest (OHCA), cardiopulmonary resuscitation (CPR), and return of spontaneous circulation (ROSC). To date, valid protein markers do not exist to prognosticate survivors and non-survivors before the end of TTM. The aim of this study is to identify specific protein patterns/arrays, which are useful for prediction in the very early phase after ROSC. Material and Methods: A total of 20 adult patients with ROSC (19 male, 1 female; 69.9 +/- 9.5 years) were included and dichotomized in two groups (survivors and non-survivors at day 30). Serum samples were drawn at day 1 after ROSC (during TTM). Three panels (organ failure, metabolic, neurology, inflammation; OLINK, Uppsala, Sweden) were utilised. A total of four proteins were found to be differentially regulated (>2- or t-test). Bioinformatic platforms were utilised to analyse pathways and identify signalling cascades and to screen for potential biomarkers. Results: A total of 276 proteins were analysed and revealed only 11 statistically significant protein alterations (Siglec-9, LAYN, SKR3, JAM-B, N2DL-2, TNF-B, BAMBI, NUCB2, STX8, PTK7, and PVLAB). Following the Bonferroni correction, no proteins were found to be regulated as statistically significant. Concerning the protein fold change for clinical significance, four proteins (IL-1 alpha, N-CDase, IL5, CRH) were found to be regulated in a clinically relevant context. Conclusions: Early analysis at 1 day after ROSC was not sufficiently possible during TTM to prognosticate survival or non-survival after OHCA. Future studies should evaluate protein expression later in the course after ROSC to identify promising protein candidates.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hinkelbein, Jochen UNSPECIFIED orcid.org/0000-0003-3585-9459 UNSPECIFIED Johnson, Lydia Kolaparambil Varghese UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiselev, Nikolai UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitz, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellmich, Martin UNSPECIFIED orcid.org/0000-0001-5174-928X UNSPECIFIED Drinhaus, Hendrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Lichtenstein, Theresa UNSPECIFIED UNSPECIFIED UNSPECIFIED Storm, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Adler, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-686572
DOI:	10.3390/jcm11040996
Journal or Publication Title:	J. Clin. Med.
Volume:	11
Number:	4
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-0383
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMERICAN-HEART-ASSOCIATION; EUROPEAN RESUSCITATION COUNCIL; CARDIOPULMONARY-RESUSCITATION; CARE; GUIDELINES; HYPOTHERMIA; UPDATE Multiple languages Medicine, General & Internal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68657