Universität zu Köln

Kroeger, Nadja, Czigany, Zoltan ORCID: 0000-0003-0373-3210, Jiang, Jipin, Afify, Mamdouh, Paschenda, Pascal, Nagai, Kazuyuki, Yagi, Shintaro and Tolba, Rene H. (2022). The Benefits of Fibrinolysis Combined with Venous Systemic Oxygen Persufflation (VSOP) in a Rat Model of Donation after Circulatory Death and Orthotopic Liver Transplantation. Int. J. Mol. Sci., 23 (9). BASEL: MDPI. ISSN 1422-0067

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Abstract

Organ shortage has led to the increasing utilization of livers retrieved from donors after circulatory death (DCD). These pre-damaged organs are susceptible to further warm ischemia and exhibit minimal tolerance for cold storage. The aim was thus to examine the effects of fibrinolysis combined with Venous Systemic Oxygen Persufflation (VSOP) on the preservation of DCD livers in vivo. Livers of male Lewis rats were explanted after 45 min of warm ischemia, cold-stored for 18 h, and transplanted into a recipient animal. Livers were left untreated or underwent either VSOP or fibrinolysis via Streptokinase (SK) or received combined SK and VSOP. Combined treatment exhibited improved microvascular flow at 168 h (p = 0.0009) and elevated microperfusion velocity at 24 h post-transplantation (p = 0.0007). Combination treatment demonstrated increased portal venous flow (PVF) at 3 and 24 h post-transplantation (p = 0.0004, p < 0.0001), although SK and VSOP analogously achieved increases at 24 h (p = 0.0036, p = 0.0051). Enzyme release was decreased for combination treatment (p = 0.0002, p = 0.0223) and lactate dehydrogenase (LDH) measurements were lower at 24 h post-transplantation (p = 0.0287). Further supporting findings have been obtained in terms of serum cytokine levels and in the alterations of endothelial injury markers. The combination treatment of SK + VSOP might provide improved organ integrity and viability and may therefore warrant further investigation as a potential therapeutic approach in the clinical setting of DCD.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kroeger, Nadja UNSPECIFIED UNSPECIFIED UNSPECIFIED Czigany, Zoltan UNSPECIFIED orcid.org/0000-0003-0373-3210 UNSPECIFIED Jiang, Jipin UNSPECIFIED UNSPECIFIED UNSPECIFIED Afify, Mamdouh UNSPECIFIED UNSPECIFIED UNSPECIFIED Paschenda, Pascal UNSPECIFIED UNSPECIFIED UNSPECIFIED Nagai, Kazuyuki UNSPECIFIED UNSPECIFIED UNSPECIFIED Yagi, Shintaro UNSPECIFIED UNSPECIFIED UNSPECIFIED Tolba, Rene H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-686764
DOI:	10.3390/ijms23095272
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	23
Number:	9
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEART-BEATING DONORS; WARM PREFLUSH; PRESERVATION; RECOMMENDATION; INJURY; TIME Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68676