Blankenagel, Sonja, Eggels, Stella ORCID: 0000-0001-9436-3109, Frey, Monika, Grill, Erwin, Bauer, Eva ORCID: 0000-0002-4820-2846, Dawid, Corinna, Fernie, Alisdair R., Haberer, Georg ORCID: 0000-0002-6612-6939, Hammerl, Richard, Barbosa Medeiros, David, Ouzunova, Milena, Presterl, Thomas, Russ, Victoria, Schaeufele, Rudi, Schlueter, Urte, Tardieu, Francois, Urbany, Claude, Urzinger, Sebastian, Weber, Andreas P. M., Schoen, Chris-Carolin and Avramova, Viktoriya ORCID: 0000-0002-6448-1312 (2022). Natural alleles of the abscisic acid catabolism gene ZmAbh4 modulate water use efficiency and carbon isotope discrimination in maize. Plant Cell, 34 (10). S. 3860 - 3873. CARY: OXFORD UNIV PRESS INC. ISSN 1532-298X

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Abstract

Altering plant water use efficiency (WUE) is a promising approach for achieving sustainable crop production in changing climate scenarios. Here, we show that WUE can be tuned by alleles of a single gene discovered in elite maize (Zea mays) breeding material. Genetic dissection of a genomic region affecting WUE led to the identification of the gene ZmAbh4 as causative for the effect. CRISPR/Cas9-mediated ZmAbh4 inactivation increased WUE without growth reductions in well-watered conditions. ZmAbh4 encodes an enzyme that hydroxylates the phytohormone abscisic acid (ABA) and initiates its catabolism. Stomatal conductance is regulated by ABA and emerged as a major link between variation in WUE and discrimination against the heavy carbon isotope (Delta C-13) during photosynthesis in the C-4 crop maize. Changes in Delta C-13 persisted in kernel material, which offers an easy-to-screen proxy for WUE. Our results establish a direct physiological and genetic link between WUE and Delta C-13 through a single gene with potential applications in maize breeding. Forward and reverse genetics identified the abscisic acid catabolism gene ZmAbh4 as a major link between variation in water use efficiency and carbon isotope discrimination through the control of stomatal conductance.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Blankenagel, SonjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eggels, StellaUNSPECIFIEDorcid.org/0000-0001-9436-3109UNSPECIFIED
Frey, MonikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grill, ErwinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, EvaUNSPECIFIEDorcid.org/0000-0002-4820-2846UNSPECIFIED
Dawid, CorinnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fernie, Alisdair R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haberer, GeorgUNSPECIFIEDorcid.org/0000-0002-6612-6939UNSPECIFIED
Hammerl, RichardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Barbosa Medeiros, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ouzunova, MilenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Presterl, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Russ, VictoriaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaeufele, RudiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schlueter, UrteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tardieu, FrancoisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Urbany, ClaudeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Urzinger, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weber, Andreas P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schoen, Chris-CarolinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Avramova, ViktoriyaUNSPECIFIEDorcid.org/0000-0002-6448-1312UNSPECIFIED
URN: urn:nbn:de:hbz:38-687213
DOI: 10.1093/plcell/koac200
Journal or Publication Title: Plant Cell
Volume: 34
Number: 10
Page Range: S. 3860 - 3873
Date: 2022
Publisher: OXFORD UNIV PRESS INC
Place of Publication: CARY
ISSN: 1532-298X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DROUGHT TOLERANCE; ABA; 8'-HYDROXYLASE; RESPONSESMultiple languages
Biochemistry & Molecular Biology; Plant Sciences; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68721

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