Universität zu Köln

Simon, Michaela, Gerlach, Roman G., Pfeifer, Yvonne, Pfennigwerth, Niels, Gatermann, Soren G., Schroeder, Agnes, Hiergeist, Andreas, Hamprecht, Axel, Ruegamer, Tamara, Gessner, Andre and Jantsch, Jonathan (2022). Increased zinc levels facilitate phenotypic detection of ceftazidime-avibactam resistance in metallo-beta-lactamase-producing Gram-negative bacteria. Front. Microbiol., 13. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

Ceftazidime-avibactam is one of the last resort antimicrobial agents for the treatment of carbapenem-resistant, Gram-negative bacteria. Metallo-beta-lactamase-producing bacteria are considered to be ceftazidime-avibactam resistant. Here, we evaluated a semi-automated antimicrobial susceptibility testing system regarding its capability to detect phenotypic ceftazidime-avibactam resistance in 176 carbapenem-resistant, metallo-beta-lactamase-producing Enterobacterales and Pseudomonas aeruginosa isolates. Nine clinical isolates displayed ceftazidime-avibactam susceptibility in the semi-automated system and six of these isolates were susceptible by broth microdilution, too. In all nine isolates, metallo-beta-lactamase-mediated hydrolytic activity was demonstrated with the EDTA-modified carbapenemase inactivation method. As zinc is known to be an important co-factor for metallo-beta-lactamase activity, test media of the semi-automated antimicrobial susceptibility testing system and broth microdilution were supplemented with zinc. Thereby, the detection of phenotypic resistance was improved in the semi-automated system and in broth microdilution. Currently, ceftazidime-avibactam is not approved as treatment option for infections by metallo-beta-lactamase-producing, Gram-negative bacteria. In infections caused by carbapenem-resistant Gram-negatives, we therefore recommend to rule out the presence of metallo-beta-lactamases with additional methods before initiating ceftazidime-avibactam treatment.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Simon, Michaela UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerlach, Roman G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeifer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfennigwerth, Niels UNSPECIFIED UNSPECIFIED UNSPECIFIED Gatermann, Soren G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, Agnes UNSPECIFIED UNSPECIFIED UNSPECIFIED Hiergeist, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruegamer, Tamara UNSPECIFIED UNSPECIFIED UNSPECIFIED Gessner, Andre UNSPECIFIED UNSPECIFIED UNSPECIFIED Jantsch, Jonathan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-689661
DOI:	10.3389/fmicb.2022.977330
Journal or Publication Title:	Front. Microbiol.
Volume:	13
Date:	2022
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENTEROBACTERIACEAE; CARBAPENEMASES Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68966