Vanacore, Adele, Vitiello, Giuseppe, Wanke, Alan, Cavasso, Domenico, Clifton, Luke A., Mahdi, Lisa, Asuncion Campanero-Rhodes, Maria, Solis, Dolores, Wuhrer, Manfred ORCID: 0000-0002-0814-4995, Nicolardi, Simone, Molinaro, Antonio, Marchetti, Roberta ORCID: 0000-0002-7173-7099, Zuccaro, Alga ORCID: 0000-0002-8026-0114, Paduano, Luigi and Silipo, Alba (2022). Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition. Carbohydr. Polym., 277. OXFORD: ELSEVIER SCI LTD. ISSN 1879-1344

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Abstract

Lipopolysaccharides, the major outer membrane components of Gram-negative bacteria, are crucial actors of the host-microbial dialogue. They can contribute to the establishment of either symbiosis or bacterial virulence, depending on the bacterial lifestyle. Plant microbiota shows great complexity, promotes plant health and growth and assures protection from pathogens. How plants perceive LPS from plant-associated bacteria and discriminate between beneficial and pathogenic microbes is an open and urgent question. Here, we report on the structure, conformation, membrane properties and immune recognition of LPS isolated from the Arabidopsis thaliana root microbiota member Herbaspirillum sp. Root189. The LPS consists of an O-methylated and variously acetylated Drhamnose containing polysaccharide with a rather hydrophobic surface. Plant immunology studies in A. thaliana demonstrate that the native acetylated O-antigen shields the LPS from immune recognition whereas the O- deacylated one does not. These findings highlight the role of Herbaspirillum LPS within plant-microbial crosstalk, and how O-antigen modifications influence membrane properties and modulate LPS host recognition.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Vanacore, AdeleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vitiello, GiuseppeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wanke, AlanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cavasso, DomenicoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clifton, Luke A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mahdi, LisaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Asuncion Campanero-Rhodes, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Solis, DoloresUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wuhrer, ManfredUNSPECIFIEDorcid.org/0000-0002-0814-4995UNSPECIFIED
Nicolardi, SimoneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Molinaro, AntonioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marchetti, RobertaUNSPECIFIEDorcid.org/0000-0002-7173-7099UNSPECIFIED
Zuccaro, AlgaUNSPECIFIEDorcid.org/0000-0002-8026-0114UNSPECIFIED
Paduano, LuigiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Silipo, AlbaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-690654
DOI: 10.1016/j.carbpol.2021.118839
Journal or Publication Title: Carbohydr. Polym.
Volume: 277
Date: 2022
Publisher: ELSEVIER SCI LTD
Place of Publication: OXFORD
ISSN: 1879-1344
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INNATE IMMUNITY; RAPID METHOD; EXTRACTION; DYNAMICS; BACTERIAMultiple languages
Chemistry, Applied; Chemistry, Organic; Polymer ScienceMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/69065

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