Srisakul, Sukrit, Wannigama, Dhammika Leshan ORCID: 0000-0001-6316-1221, Higgins, Paul G., Hurst, Cameron ORCID: 0000-0002-4577-8962, Abe, Shuichi, Hongsing, Parichart, Saethang, Thammakorn, Luk-in, Sirirat, Liao, Tingting, Kueakulpattana, Naris, Shein, Aye Mya Sithu, Gan, Lin, Kupwiwat, Rosalyn, Tanasatitchai, Chanikan, Wapeesittipan, Pattama, Phattharapornjaroen, Phatthranit, Badavath, Vishnu Nayak, Leelahavanichkul, Asada and Chatsuwan, Tanittha (2022). Overcoming addition of phosphoethanolamine to lipid A mediated colistin resistance in Acinetobacter baumannii clinical isolates with colistin-sulbactam combination therapy. Sci Rep, 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

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Abstract

Overcoming colistin-resistant Acinetobacter baumannii (CoR-AB) has become a major concern due to the lack of effective antibiotics. This study aimed to explore the prevalence of CoR-AB clinical isolates in Thailand, their mechanisms of resistance, and test the efficacy of colistin plus sulbactam against CoR-AB isolates. The colistin resistance rate among carbapenem-resistant A. baumannii was 15.14%. The mcr gene or its variants were not detected in CoR-AB isolates by PCR screening. The lipid A mass spectra of CoR-AB isolates showed the additional [M-H](-) ion peak at m/z = 2034 that correlated to the phosphoethanolamine (pEtN) addition to lipid A (N = 27/30). The important amino acid substitutions were found at position S14P, A138T, A227V in PmrB that are associated with overexpression of the pEtN transferase (PmrC) and contributed the pEtN addition. The lipopolysacccharide production genes (lpxACD) were not related to lipid A mass spectra. A colistin plus sulbactam combination exhibited the synergy rate at 86.7% against CoR-AB isolates compare to sulbactam (85.89% resistance) or colistin (15.14% resistance) alone. The excellent synergistic activity of colistin plus sulbactam combination has the potential for the treatment of CoR-AB infections.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Srisakul, SukritUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wannigama, Dhammika LeshanUNSPECIFIEDorcid.org/0000-0001-6316-1221UNSPECIFIED
Higgins, Paul G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hurst, CameronUNSPECIFIEDorcid.org/0000-0002-4577-8962UNSPECIFIED
Abe, ShuichiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hongsing, ParichartUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saethang, ThammakornUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Luk-in, SiriratUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liao, TingtingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kueakulpattana, NarisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shein, Aye Mya SithuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gan, LinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kupwiwat, RosalynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tanasatitchai, ChanikanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wapeesittipan, PattamaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Phattharapornjaroen, PhatthranitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Badavath, Vishnu NayakUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leelahavanichkul, AsadaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chatsuwan, TanitthaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-694456
DOI: 10.1038/s41598-022-15386-1
Journal or Publication Title: Sci Rep
Volume: 12
Number: 1
Date: 2022
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INTENSIVE-CARE-UNIT; ANTIMICROBIAL SUSCEPTIBILITY; MULTIDRUG-RESISTANT; MECHANISMS; LIPOPOLYSACCHARIDE; PMRAB; IDENTIFICATION; CALCOACETICUS; POLYMYXINSMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/69445

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