Dewald, Felix, Suarez, Isabelle, Johnen, Ronja ORCID: 0000-0002-5150-5266, Grossbach, Jan ORCID: 0000-0002-9394-5665, Moran-Tovar, Roberto ORCID: 0000-0002-6291-9376, Steger, Gertrud, Joachim, Alexander, Rubio, Gibran Horemheb, Fries, Mira, Behr, Florian, Kley, Joao, Lingnau, Andreas, Kretschmer, Alina, Gude, Carina, Beazes-Flores, Guadelupe, del Valle, David Laveaga, Roblero-Hernandez, Alberto, Magana-Cerino, Jesus, Hernandez, Adriana Torres, Ruiz-Quinones, Jesus, Schega, Konstantin, Linne, Viktoria, Junker, Lena, Wunsch, Marie ORCID: 0000-0002-6570-1831, Heger, Eva, Knops, Elena, Di Cristanziano, Veronica, Meyer, Meike ORCID: 0000-0002-5708-3981, Huenseler, Christoph, Weber, Lutz T., Lueers, Jan-Christoffer, Quade, Gustav, Wisplinghoff, Hilmar, Tiemann, Carsten, Zotz, Rainer, Jomaa, Hassan, Pranada, Arthur, Herzum, Ileana, Cullen, Paul, Schmitz, Franz-Josef, Philipsen, Paul, Kirchner, Georg, Knabbe, Cornelius, Hellmich, Martin, Buess, Michael, Wolff, Anna, Kossow, Annelene, Niessen, Johannes, Jeworutzki, Sebastian ORCID: 0000-0002-2671-5253, Schraepler, Joerg-Peter, Laessig, Michael, Doetsch, Joerg, Faetkenheuer, Gerd, Kaiser, Rolf ORCID: 0000-0002-2640-2548, Beyer, Andreas, Rybniker, Jan and Klein, Florian ORCID: 0000-0003-1376-1792 (2022). Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children. Nat. Commun., 13 (1). BERLIN: NATURE PORTFOLIO. ISSN 2041-1723

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Abstract

Systematic SARS-CoV-2 testing is a valuable tool for infection control and surveillance. However, broad application of high sensitive RT-qPCR testing in children is often hampered due to unpleasant sample collection, limited RT-qPCR capacities and high costs. Here, we developed a high-throughput approach ('Lolli-Method') for SARS-CoV-2 detection in children, combining non-invasive sample collection with an RT-qPCR-pool testing strategy. SARS-CoV-2 infections were diagnosed with sensitivities of 100% and 93.9% when viral loads were >10(6) copies/ml and >10(3) copies/ml in corresponding Naso-/Oropharyngeal-swabs, respectively. For effective application of the Lolli-Method in schools and daycare facilities, SEIR-modeling indicated a preferred frequency of two tests per week. The developed test strategy was implemented in 3,700 schools and 698 daycare facilities in Germany, screening over 800,000 individuals twice per week. In a period of 3 months, 6,364 pool-RT-qPCRs tested positive (0.64%), ranging from 0.05% to 2.61% per week. Notably, infections correlated with local SARS-CoV-2 incidences and with a school social deprivation index. Moreover, in comparison with the alpha variant, statistical modeling revealed a 36.8% increase for multiple (>= 2 children) infections per class following infections with the delta variant. We conclude that the Lolli-Method is a powerful tool for SARS-CoV-2 surveillance and can support infection control in schools and daycare facilities. Dewald et al. combine a non-invasive sampling approach (Lolli-Test) with an RT qPCR-pool testing strategy to screen for SARS-CoV-2 infections in children and use the method for surveillance and infection control in > 4000 school and daycare settings.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Dewald, FelixUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Suarez, IsabelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Johnen, RonjaUNSPECIFIEDorcid.org/0000-0002-5150-5266UNSPECIFIED
Grossbach, JanUNSPECIFIEDorcid.org/0000-0002-9394-5665UNSPECIFIED
Moran-Tovar, RobertoUNSPECIFIEDorcid.org/0000-0002-6291-9376UNSPECIFIED
Steger, GertrudUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Joachim, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rubio, Gibran HoremhebUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fries, MiraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Behr, FlorianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kley, JoaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lingnau, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kretschmer, AlinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gude, CarinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beazes-Flores, GuadelupeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
del Valle, David LaveagaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roblero-Hernandez, AlbertoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Magana-Cerino, JesusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hernandez, Adriana TorresUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ruiz-Quinones, JesusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schega, KonstantinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Linne, ViktoriaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Junker, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wunsch, MarieUNSPECIFIEDorcid.org/0000-0002-6570-1831UNSPECIFIED
Heger, EvaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knops, ElenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Di Cristanziano, VeronicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meyer, MeikeUNSPECIFIEDorcid.org/0000-0002-5708-3981UNSPECIFIED
Huenseler, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weber, Lutz T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lueers, Jan-ChristofferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Quade, GustavUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wisplinghoff, HilmarUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tiemann, CarstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zotz, RainerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jomaa, HassanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pranada, ArthurUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herzum, IleanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cullen, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmitz, Franz-JosefUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Philipsen, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kirchner, GeorgUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knabbe, CorneliusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hellmich, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buess, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wolff, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kossow, AnneleneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niessen, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jeworutzki, SebastianUNSPECIFIEDorcid.org/0000-0002-2671-5253UNSPECIFIED
Schraepler, Joerg-PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Laessig, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doetsch, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Faetkenheuer, GerdUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kaiser, RolfUNSPECIFIEDorcid.org/0000-0002-2640-2548UNSPECIFIED
Beyer, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rybniker, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klein, FlorianUNSPECIFIEDorcid.org/0000-0003-1376-1792UNSPECIFIED
URN: urn:nbn:de:hbz:38-694541
DOI: 10.1038/s41467-022-30664-2
Journal or Publication Title: Nat. Commun.
Volume: 13
Number: 1
Date: 2022
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
COVID-19Multiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/69454

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