Universität zu Köln

Opazo-Capurro, Andres ORCID: 0000-0002-6382-7625, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Wille, Julia, Seifert, Harald, Cigarroa, Camila, Gonzalez-Munoz, Paulina, Quezada-Aguiluz, Mario ORCID: 0000-0001-9644-8368, Dominguez-Yevenes, Mariana, Bello-Toledo, Helia, Vergara, Luis and Gonzalez-Rocha, Gerardo (2019). Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes. Front. Cell. Infect. Microbiol., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988

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Abstract

While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (ICS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic bla(OXA-)(23-like) gene, A154 harbored the plasmid-encoded antibiotic-resistance genes bla(PER-2), tet(B), aph(3')-VIa, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Opazo-Capurro, Andres UNSPECIFIED orcid.org/0000-0002-6382-7625 UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Cigarroa, Camila UNSPECIFIED UNSPECIFIED UNSPECIFIED Gonzalez-Munoz, Paulina UNSPECIFIED UNSPECIFIED UNSPECIFIED Quezada-Aguiluz, Mario UNSPECIFIED orcid.org/0000-0001-9644-8368 UNSPECIFIED Dominguez-Yevenes, Mariana UNSPECIFIED UNSPECIFIED UNSPECIFIED Bello-Toledo, Helia UNSPECIFIED UNSPECIFIED UNSPECIFIED Vergara, Luis UNSPECIFIED UNSPECIFIED UNSPECIFIED Gonzalez-Rocha, Gerardo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-141402
DOI:	10.3389/fcimb.2019.00328
Journal or Publication Title:	Front. Cell. Infect. Microbiol.
Volume:	9
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	2235-2988
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BETA-LACTAMASE; CARBAPENEM RESISTANCE; IDENTIFICATION; PLASMIDS; SCO-1 Multiple languages Immunology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14140