Opazo-Capurro, Andres ORCID: 0000-0002-6382-7625, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Wille, Julia, Seifert, Harald, Cigarroa, Camila, Gonzalez-Munoz, Paulina, Quezada-Aguiluz, Mario ORCID: 0000-0001-9644-8368, Dominguez-Yevenes, Mariana, Bello-Toledo, Helia, Vergara, Luis and Gonzalez-Rocha, Gerardo (2019). Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes. Front. Cell. Infect. Microbiol., 9. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988

Full text not available from this repository.

Abstract

While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (ICS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic bla(OXA-)(23-like) gene, A154 harbored the plasmid-encoded antibiotic-resistance genes bla(PER-2), tet(B), aph(3')-VIa, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Opazo-Capurro, AndresUNSPECIFIEDorcid.org/0000-0002-6382-7625UNSPECIFIED
Higgins, Paul G.UNSPECIFIEDorcid.org/0000-0001-8677-9454UNSPECIFIED
Wille, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seifert, HaraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cigarroa, CamilaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gonzalez-Munoz, PaulinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Quezada-Aguiluz, MarioUNSPECIFIEDorcid.org/0000-0001-9644-8368UNSPECIFIED
Dominguez-Yevenes, MarianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bello-Toledo, HeliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vergara, LuisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gonzalez-Rocha, GerardoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-141402
DOI: 10.3389/fcimb.2019.00328
Journal or Publication Title: Front. Cell. Infect. Microbiol.
Volume: 9
Date: 2019
Publisher: FRONTIERS MEDIA SA
Place of Publication: LAUSANNE
ISSN: 2235-2988
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BETA-LACTAMASE; CARBAPENEM RESISTANCE; IDENTIFICATION; PLASMIDS; SCO-1Multiple languages
Immunology; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/14140

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item