Universität zu Köln

Hueynck, Jan Schulze, Kaschani, Farnusch, van der Linde, Karina ORCID: 0000-0003-1390-8344, Ziemann, Sebastian, Mueller, Andre N., Colby, Thomas, Kaiser, Markus, Villamil, Johana C. Misas and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2019). Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity. Front. Plant Sci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

Plant proteases are key regulators of plant cell processes such as seed development, immune responses, senescence and programmed cell death (PCD). Apoplastic papain-like cysteine proteases (PL) are hubs in plant-microbe interactions and play an important role during abiotic stresses. The apoplast is a crucial interface for the interaction between plant and microbes. So far, apoplastic maize PL and their function have been mostly described for aerial parts. In this study, we focused on apoplastic PLCPs in the roots of maize plants. We have analyzed the phylogeny of maize PLCPs and investigated their protein abundance after salicylic acid (SA) treatment. Using activity-based protein profiling (ABPP) we have identified a novel root-specific PLCP belonging to the RD21-like subfamily, as well as three SA activated PLCPs. The root specific PLCP CP1C shares sequence and structural similarities to known CP1-like proteases. Biochemical analysis of recombinant CP1C revealed different substrate specificities and inhibitor affinities compared to the related proteases. This study characterized a root-specific PLCP and identifies differences between the SA-dependent activation of PLCPs in roots and leaves.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hueynck, Jan Schulze UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaschani, Farnusch UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Linde, Karina UNSPECIFIED orcid.org/0000-0003-1390-8344 UNSPECIFIED Ziemann, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Andre N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Colby, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Villamil, Johana C. Misas UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-150253
DOI:	10.3389/fpls.2019.00473
Journal or Publication Title:	Front. Plant Sci.
Volume:	10
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CATHEPSIN-B; PHYTOPHTHORA-INFESTANS; C1A CYSTEINE; GENE FAMILY; CELL-DEATH; ARABIDOPSIS; INHIBITORS; SENESCENCE; CYSTATIN; PROTEOME Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15025