Universität zu Köln

Okmen, Bilal, Mathow, Daniel, Hof, Alexander, Lahrmann, Urs, Assmann, Daniela and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2018). Mining the effector repertoire of the biotrophic fungal pathogen Ustilago hordei during host and non-host infection. Mol. Plant Pathol., 19 (12). S. 2603 - 2623. HOBOKEN: WILEY. ISSN 1364-3703

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Abstract

The success of plant-pathogenic fungi mostly relies on their arsenal of virulence factors which are expressed and delivered into the host tissue during colonization. The biotrophic fungal pathogen Ustilago hordei causes covered smut disease on both barley and oat. In this study, we combined cytological, genomics and molecular biological methods to achieve a better understanding of the molecular interactions in the U. hordei-barley pathosystem. Microscopic analysis revealed that U. hordei densely colonizes barley leaves on penetration, in particular the vascular system. Transcriptome analysis of U. hordei at different stages of host infection revealed differential expression of the transcript levels of 273 effector gene candidates. Furthermore, U. hordei transcriptionally activates core effector genes which may suppress even non-host early defence responses. Based on expression profiles and novelty of sequences, knockout studies of 14 effector candidates were performed in U. hordei, which resulted in the identification of four virulence factors required for host colonization. Yeast two-hybrid screening identified potential barley targets for two of the effectors. Overall, this study provides a first systematic analysis of the effector repertoire of U. hordei and identifies four effectors (Uvi1-Uvi4) as virulence factors for the infection of barley.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Okmen, Bilal UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathow, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Hof, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Lahrmann, Urs UNSPECIFIED UNSPECIFIED UNSPECIFIED Assmann, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-164101
DOI:	10.1111/mpp.12732
Journal or Publication Title:	Mol. Plant Pathol.
Volume:	19
Number:	12
Page Range:	S. 2603 - 2623
Date:	2018
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1364-3703
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHITIN-TRIGGERED IMMUNITY; CELL-DEATH SUPPRESSOR; PHYTOPHTHORA-INFESTANS; DISEASE RESISTANCE; MAGNAPORTHE-ORYZAE; BAX INHIBITOR-1; PROTEASE INHIBITOR; ANTIFUNGAL PROTEIN; SMUT FUNGUS; MAYDIS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16410