Paulsson, Mats and Wagener, Raimund (2018). Matrilins. In: Methods in Cell Biology, S. 429 - 447. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC. ISBN 978-0-12-812297-6
Full text not available from this repository.Abstract
Marilins mediate interactions between macromolecular components of the extracellular matrix, e.g., collagens and proteoglycans. They are composed of von Willebrand factor type A and epidermal growth factor-like domains and the subunits oligomerize via coiled-coil domains. Matrilin-1 and -3 are abundant in hyaline cartilage, whereas matrilin-2 and -4 are widespread but less abundant. Mutations in matrilin genes have been linked to chondrodysplasias and osteoarthritis and recently characterization of matrilin-deficient mice revealed novel functions in mechanotransduction, regeneration, or inflammation. Due to their intrinsic adhesiveness and partially also low abundance, the study of matrilins is cumbersome. In this chapter, we describe methods for purification of matrilins from tissue, analysis of matrilins in tissue extracts, recombinant expression, and generation of matrilin-specific antibodies.
Item Type: | Book Section, Proceedings Item or annotation in a legal commentary | ||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-202656 | ||||||||||||
DOI: | 10.1016/bs.mcb.2017.08.018 | ||||||||||||
Title of Book: | Methods in Cell Biology | ||||||||||||
Series Name: | Methods Cell Biol. | ||||||||||||
Volume: | 143 | ||||||||||||
Page Range: | S. 429 - 447 | ||||||||||||
Date: | 2018 | ||||||||||||
Publisher: | ELSEVIER ACADEMIC PRESS INC | ||||||||||||
Place of Publication: | SAN DIEGO | ||||||||||||
ISSN: | 0091-679X | ||||||||||||
ISBN: | 978-0-12-812297-6 | ||||||||||||
Language: | English | ||||||||||||
Faculty: | Unspecified | ||||||||||||
Divisions: | Unspecified | ||||||||||||
Subjects: | no entry | ||||||||||||
Uncontrolled Keywords: |
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Refereed: | Yes | ||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/20265 |
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