Universität zu Köln

Gedik, Nilguen, Krueger, Marcus ORCID: 0000-0003-2008-4582, Thielmann, Matthias, Kottenberg, Eva, Skyschally, Andreas, Frey, Ulrich H., Cario, Elke, Peters, Juergen, Jakob, Heinz, Heusch, Gerd and Kleinbongard, Petra (2017). Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning. Sci Rep, 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Remote ischemic preconditioning (RIPC) by repeated brief cycles of limb ischemia/reperfusion reduces myocardial ischemia/reperfusion injury. In left ventricular (LV) biopsies from patients undergoing coronary artery bypass grafting (CABG), only the activation of signal transducer and activator of transcription 5 was associated with RIPC's cardioprotection. We have now used an unbiased, non-hypothesis-driven proteomics and phosphoproteomics approach to analyze LV biopsies from patients undergoing CABG and from pigs undergoing coronary occlusion/reperfusion without (sham) and with RIPC. False discovery rate-based statistics identified a higher prostaglandin reductase 2 expression at early reperfusion with RIPC than with sham in patients. In pigs, the phosphorylation of 116 proteins was different between baseline and early reperfusion with RIPC and/or with sham. The identified proteins were not identical for patients and pigs, but in-silico pathway analysis of proteins with >= 2-fold higher expression/phosphorylation at early reperfusion with RIPC in comparison to sham revealed a relation to mitochondria and cytoskeleton in both species. Apart from limitations of the proteomics analysis per se, the small cohorts, the sampling/sample processing and the number of uncharacterized/unverifiable porcine proteins may have contributed to this largely unsatisfactory result.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gedik, Nilguen UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Thielmann, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Kottenberg, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Skyschally, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Frey, Ulrich H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cario, Elke UNSPECIFIED UNSPECIFIED UNSPECIFIED Peters, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakob, Heinz UNSPECIFIED UNSPECIFIED UNSPECIFIED Heusch, Gerd UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleinbongard, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221878
DOI:	10.1038/s41598-017-07883-5
Journal or Publication Title:	Sci Rep
Volume:	7
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ELEVATION MYOCARDIAL-INFARCTION; PRESERVES MITOCHONDRIAL-FUNCTION; CARDIOLOGY WORKING GROUP; INJURY SALVAGE KINASE; BYPASS GRAFT-SURGERY; K-ATP CHANNELS; REPERFUSION INJURY; ISCHEMIA/REPERFUSION INJURY; SIGNAL-TRANSDUCTION; CALPAIN ACTIVATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22187