Berninger, Markus T., Mohajerani, Pouyan, Wildgruber, Moritz, Beziere, Nicolas ORCID: 0000-0002-2076-7060, Kimm, Melanie A., Ma, Xiaopeng, Haller, Bernhard ORCID: 0000-0002-9723-393X, Fleming, Megan J., Vogt, Stephan, Anton, Martina, Imhoff, Andreas B., Ntziachristos, Vasilis, Meier, Reinhard ORCID: 0000-0002-2169-4780 and Henning, Tobias D. (2017). Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography. Photoacoustics, 6. S. 37 - 48. MUNICH: ELSEVIER GMBH. ISSN 2213-5979

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Abstract

The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT-and Caspase-3/-7-testing in vitro. 2 x 10(6), 2 x 10(5) and 2 x 10(4) MSCs labeled with 5 and 10 mu g DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 mu g DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model. (C) 2017 The Authors. Published by Elsevier GmbH.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Berninger, Markus T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mohajerani, PouyanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wildgruber, MoritzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beziere, NicolasUNSPECIFIEDorcid.org/0000-0002-2076-7060UNSPECIFIED
Kimm, Melanie A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ma, XiaopengUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haller, BernhardUNSPECIFIEDorcid.org/0000-0002-9723-393XUNSPECIFIED
Fleming, Megan J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vogt, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Anton, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Imhoff, Andreas B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ntziachristos, VasilisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meier, ReinhardUNSPECIFIEDorcid.org/0000-0002-2169-4780UNSPECIFIED
Henning, Tobias D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-228081
DOI: 10.1016/j.pacs.2017.04.002
Journal or Publication Title: Photoacoustics
Volume: 6
Page Range: S. 37 - 48
Date: 2017
Publisher: ELSEVIER GMBH
Place of Publication: MUNICH
ISSN: 2213-5979
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ACUTE MYOCARDIAL-INFARCTION; BONE-MARROW-CELLS; IN-VIVO; PROGENITOR CELLS; CARDIAC-FUNCTION; GOLD NANORODS; TRANSPLANTATION; FLUORESCENCE; SURVIVAL; MODELMultiple languages
Acoustics; Engineering, Biomedical; Instruments & Instrumentation; Radiology, Nuclear Medicine & Medical ImagingMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22808

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