Universität zu Köln

Berninger, Markus T., Mohajerani, Pouyan, Wildgruber, Moritz, Beziere, Nicolas ORCID: 0000-0002-2076-7060, Kimm, Melanie A., Ma, Xiaopeng, Haller, Bernhard ORCID: 0000-0002-9723-393X, Fleming, Megan J., Vogt, Stephan, Anton, Martina, Imhoff, Andreas B., Ntziachristos, Vasilis, Meier, Reinhard ORCID: 0000-0002-2169-4780 and Henning, Tobias D. (2017). Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography. Photoacoustics, 6. S. 37 - 48. MUNICH: ELSEVIER GMBH. ISSN 2213-5979

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Abstract

The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT-and Caspase-3/-7-testing in vitro. 2 x 10(6), 2 x 10(5) and 2 x 10(4) MSCs labeled with 5 and 10 mu g DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 mu g DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model. (C) 2017 The Authors. Published by Elsevier GmbH.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Berninger, Markus T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohajerani, Pouyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wildgruber, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Beziere, Nicolas UNSPECIFIED orcid.org/0000-0002-2076-7060 UNSPECIFIED Kimm, Melanie A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ma, Xiaopeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Haller, Bernhard UNSPECIFIED orcid.org/0000-0002-9723-393X UNSPECIFIED Fleming, Megan J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogt, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Anton, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Imhoff, Andreas B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ntziachristos, Vasilis UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Reinhard UNSPECIFIED orcid.org/0000-0002-2169-4780 UNSPECIFIED Henning, Tobias D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-228081
DOI:	10.1016/j.pacs.2017.04.002
Journal or Publication Title:	Photoacoustics
Volume:	6
Page Range:	S. 37 - 48
Date:	2017
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	2213-5979
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACUTE MYOCARDIAL-INFARCTION; BONE-MARROW-CELLS; IN-VIVO; PROGENITOR CELLS; CARDIAC-FUNCTION; GOLD NANORODS; TRANSPLANTATION; FLUORESCENCE; SURVIVAL; MODEL Multiple languages Acoustics; Engineering, Biomedical; Instruments & Instrumentation; Radiology, Nuclear Medicine & Medical Imaging Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22808