Universität zu Köln

Shimabukuro-Vornhagen, Alexander ORCID: 0000-0002-2351-7294, Garcia-Marquez, Maria Alejandra ORCID: 0000-0002-5923-5942, Fischer, Rieke N., Iltgen-Breburda, Juliane, Fiedler, Anne, Wennhold, Kerstin ORCID: 0000-0001-5539-7226, Rappl, Gunter ORCID: 0000-0003-0402-7940, Abken, Hinrich ORCID: 0000-0002-4302-3240, Lehmann, Clara ORCID: 0000-0002-7042-1578, Herling, Marco, Wolf, Dominik, Fätkenheuer, Gerd ORCID: 0000-0002-5054-1871, Rubbert-Roth, Andrea ORCID: 0000-0002-9016-2833, Hallek, Michael ORCID: 0000-0002-7425-4455, Theurich, Sebastian ORCID: 0000-0001-5706-8258 and von Bergwelt-Baildon, Michael (2017). Antigen-presenting human B cells are expanded in inflammatory conditions. J. Leukoc. Biol., 101 (2). S. 577 - 588. HOBOKEN: WILEY. ISSN 1938-3673

Full text not available from this repository.

Abstract

Traditionally, B cells have been best known for their role as producers of antibodies. However, in recent years, a growing body of evidence has accumulated showing that B cells fulfill a range of other immunologic functions. One of the functions that has attracted increasing attention is the capacity of B cells to induce antigen-specific activation of T cells through presentation of antigens. However, the analysis of this B cell function has been hampered by the lack of a phenotypically well-defined antigen-presenting B cell subset. Here, we report the identification of a human antigen-presenting B cell subset with strong immunostimulatory properties. This B cell subset is characterized by low expression of CD21 and high expression of the activation marker CD86 and exhibits strong T cell-stimulatory activity, as demonstrated by means of an autologous mixed-lymphocyte reaction. Phenotypically, CD21(low)CD86(pos) immunostimulatory B cells (B-APC) represented CD27(+) class-switched IgM(neg)IgD(neg) B lymphocytes and displayed a higher expression of cell surface receptors, which mediate the migration from peripheral blood to sites of inflammation. Flow cytometric analysis of peripheral blood obtained from individuals with inflammatory conditions revealed that the B-APC subset was expanded following vaccination and in patients with rheumatoid arthritis. Taken together, our work shows that B-APC represents a strongly immunostimulatory B cell subset, which could be a promising target for immunotherapeutic intervention in inflammatory diseases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shimabukuro-Vornhagen, Alexander UNSPECIFIED orcid.org/0000-0002-2351-7294 UNSPECIFIED Garcia-Marquez, Maria Alejandra UNSPECIFIED orcid.org/0000-0002-5923-5942 UNSPECIFIED Fischer, Rieke N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Iltgen-Breburda, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Fiedler, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Wennhold, Kerstin UNSPECIFIED orcid.org/0000-0001-5539-7226 UNSPECIFIED Rappl, Gunter UNSPECIFIED orcid.org/0000-0003-0402-7940 UNSPECIFIED Abken, Hinrich UNSPECIFIED orcid.org/0000-0002-4302-3240 UNSPECIFIED Lehmann, Clara UNSPECIFIED orcid.org/0000-0002-7042-1578 UNSPECIFIED Herling, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED Fätkenheuer, Gerd UNSPECIFIED orcid.org/0000-0002-5054-1871 UNSPECIFIED Rubbert-Roth, Andrea UNSPECIFIED orcid.org/0000-0002-9016-2833 UNSPECIFIED Hallek, Michael UNSPECIFIED orcid.org/0000-0002-7425-4455 UNSPECIFIED Theurich, Sebastian UNSPECIFIED orcid.org/0000-0001-5706-8258 UNSPECIFIED von Bergwelt-Baildon, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-241737
DOI:	10.1189/jlb.5A0416-182R
Journal or Publication Title:	J. Leukoc. Biol.
Volume:	101
Number:	2
Page Range:	S. 577 - 588
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1938-3673
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Sonstiges > Zentrum für Versorgungsforschung Köln
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language FC-GAMMA-RIIB; DENDRITIC CELLS; ACTIVATION; ARTHRITIS; CONTAIN; AUTOIMMUNITY; LYMPHOCYTES; REGULATORS; EXPRESSION; RECEPTORS Multiple languages Cell Biology; Hematology; Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24173