Shimabukuro-Vornhagen, Alexander, Garcia-Marquez, Maria, Fischer, Rieke N., Iltgen-Breburda, Juliane, Fiedler, Anne, Wennhold, Kerstin ORCID: 0000-0001-5539-7226, Rappl, Gunter, Abken, Hinrich, Lehmann, Clara, Herling, Marco, Wolf, Dominik, Faetkenheuer, Gerd, Rubbert-Roth, Andrea, Hallek, Michael, Theurich, Sebastian ORCID: 0000-0001-5706-8258 and von Bergwelt-Baildon, Michael (2017). Antigen-presenting human B cells are expanded in inflammatory conditions. J. Leukoc. Biol., 101 (2). S. 577 - 588. HOBOKEN: WILEY. ISSN 1938-3673

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Abstract

Traditionally, B cells have been best known for their role as producers of antibodies. However, in recent years, a growing body of evidence has accumulated showing that B cells fulfill a range of other immunologic functions. One of the functions that has attracted increasing attention is the capacity of B cells to induce antigen-specific activation of T cells through presentation of antigens. However, the analysis of this B cell function has been hampered by the lack of a phenotypically well-defined antigen-presenting B cell subset. Here, we report the identification of a human antigen-presenting B cell subset with strong immunostimulatory properties. This B cell subset is characterized by low expression of CD21 and high expression of the activation marker CD86 and exhibits strong T cell-stimulatory activity, as demonstrated by means of an autologous mixed-lymphocyte reaction. Phenotypically, CD21(low)CD86(pos) immunostimulatory B cells (B-APC) represented CD27(+) class-switched IgM(neg)IgD(neg) B lymphocytes and displayed a higher expression of cell surface receptors, which mediate the migration from peripheral blood to sites of inflammation. Flow cytometric analysis of peripheral blood obtained from individuals with inflammatory conditions revealed that the B-APC subset was expanded following vaccination and in patients with rheumatoid arthritis. Taken together, our work shows that B-APC represents a strongly immunostimulatory B cell subset, which could be a promising target for immunotherapeutic intervention in inflammatory diseases.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Shimabukuro-Vornhagen, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Garcia-Marquez, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fischer, Rieke N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Iltgen-Breburda, JulianeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fiedler, AnneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wennhold, KerstinUNSPECIFIEDorcid.org/0000-0001-5539-7226UNSPECIFIED
Rappl, GunterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Abken, HinrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lehmann, ClaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herling, MarcoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wolf, DominikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Faetkenheuer, GerdUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rubbert-Roth, AndreaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hallek, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Theurich, SebastianUNSPECIFIEDorcid.org/0000-0001-5706-8258UNSPECIFIED
von Bergwelt-Baildon, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-241737
DOI: 10.1189/jlb.5A0416-182R
Journal or Publication Title: J. Leukoc. Biol.
Volume: 101
Number: 2
Page Range: S. 577 - 588
Date: 2017
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1938-3673
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
FC-GAMMA-RIIB; DENDRITIC CELLS; ACTIVATION; ARTHRITIS; CONTAIN; AUTOIMMUNITY; LYMPHOCYTES; REGULATORS; EXPRESSION; RECEPTORSMultiple languages
Cell Biology; Hematology; ImmunologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/24173

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