Universität zu Köln

Aarabi, Fayezeh ORCID: 0000-0002-5099-5400, Kusajima, Miyuki, Tohge, Takayuki, Konishi, Tomokazu ORCID: 0000-0003-4826-6265, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Takamune, Makiko, Sasazaki, Yoko, Watanabe, Mutsumi, Nakashita, Hideo, Fernie, Alisdair R., Saito, Kazuki ORCID: 0000-0001-6310-5342, Takahashi, Hideki ORCID: 0000-0001-8439-0533, Hubberten, Hans-Michael, Hoefgen, Rainer and Maruyama-Nakashita, Akiko ORCID: 0000-0002-3240-2843 (2016). Sulfur deficiency-induced repressor proteins optimize glucosinolate biosynthesis in plants. Sci. Adv., 2 (10). WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 2375-2548

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Abstract

Glucosinolates (GSLs) in the plant order of the Brassicales are sulfur-rich secondary metabolites that harbor anti-pathogenic and antiherbivory plant-protective functions and have medicinal properties, such as carcinopreventive and antibiotic activities. Plants repress GSL biosynthesis upon sulfur deficiency (-S); hence, field performance and medicinal quality are impaired by inadequate sulfate supply. The molecular mechanism that links -S to GSL biosynthesis has remained understudied. We report here the identification of the -S marker genes sulfur deficiency induced 1 (SDI1) and SDI2 acting as major repressors controlling GSL biosynthesis in Arabidopsis under -S condition. SDI1 and SDI2 expression negatively correlated with GSL biosynthesis in both transcript and metabolite levels. Principal components analysis of transcriptome data indicated that SDI1 regulates aliphatic GSL biosynthesis as part of -S response. SDI1 was localized to the nucleus and interacted with MYB28, a major transcription factor that promotes aliphatic GSL biosynthesis, in both yeast and plant cells. SDI1 inhibited the transcription of aliphatic GSL biosynthetic genes by maintaining the DNA binding composition in the form of an SDI1-MYB28 complex, leading to down-regulation of GSL biosynthesis and prioritization of sulfate usage for primary metabolites under sulfur-deprived conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Aarabi, Fayezeh UNSPECIFIED orcid.org/0000-0002-5099-5400 UNSPECIFIED Kusajima, Miyuki UNSPECIFIED UNSPECIFIED UNSPECIFIED Tohge, Takayuki UNSPECIFIED UNSPECIFIED UNSPECIFIED Konishi, Tomokazu UNSPECIFIED orcid.org/0000-0003-4826-6265 UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Takamune, Makiko UNSPECIFIED UNSPECIFIED UNSPECIFIED Sasazaki, Yoko UNSPECIFIED UNSPECIFIED UNSPECIFIED Watanabe, Mutsumi UNSPECIFIED UNSPECIFIED UNSPECIFIED Nakashita, Hideo UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Saito, Kazuki UNSPECIFIED orcid.org/0000-0001-6310-5342 UNSPECIFIED Takahashi, Hideki UNSPECIFIED orcid.org/0000-0001-8439-0533 UNSPECIFIED Hubberten, Hans-Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoefgen, Rainer UNSPECIFIED UNSPECIFIED UNSPECIFIED Maruyama-Nakashita, Akiko UNSPECIFIED orcid.org/0000-0002-3240-2843 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-261859
DOI:	10.1126/sciadv.1601087
Journal or Publication Title:	Sci. Adv.
Volume:	2
Number:	10
Date:	2016
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	2375-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BHLH TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; SYSTEMS BIOLOGY; GENE-EXPRESSION; SULFATE UPTAKE; REGULATOR; MYB; METABOLOMICS; TRANSPORTERS; ASSIMILATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26185