Aarabi, Fayezeh ORCID: 0000-0002-5099-5400, Kusajima, Miyuki, Tohge, Takayuki, Konishi, Tomokazu ORCID: 0000-0003-4826-6265, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Takamune, Makiko, Sasazaki, Yoko, Watanabe, Mutsumi, Nakashita, Hideo, Fernie, Alisdair R., Saito, Kazuki ORCID: 0000-0001-6310-5342, Takahashi, Hideki ORCID: 0000-0001-8439-0533, Hubberten, Hans-Michael, Hoefgen, Rainer and Maruyama-Nakashita, Akiko ORCID: 0000-0002-3240-2843 (2016). Sulfur deficiency-induced repressor proteins optimize glucosinolate biosynthesis in plants. Sci. Adv., 2 (10). WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 2375-2548

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Abstract

Glucosinolates (GSLs) in the plant order of the Brassicales are sulfur-rich secondary metabolites that harbor anti-pathogenic and antiherbivory plant-protective functions and have medicinal properties, such as carcinopreventive and antibiotic activities. Plants repress GSL biosynthesis upon sulfur deficiency (-S); hence, field performance and medicinal quality are impaired by inadequate sulfate supply. The molecular mechanism that links -S to GSL biosynthesis has remained understudied. We report here the identification of the -S marker genes sulfur deficiency induced 1 (SDI1) and SDI2 acting as major repressors controlling GSL biosynthesis in Arabidopsis under -S condition. SDI1 and SDI2 expression negatively correlated with GSL biosynthesis in both transcript and metabolite levels. Principal components analysis of transcriptome data indicated that SDI1 regulates aliphatic GSL biosynthesis as part of -S response. SDI1 was localized to the nucleus and interacted with MYB28, a major transcription factor that promotes aliphatic GSL biosynthesis, in both yeast and plant cells. SDI1 inhibited the transcription of aliphatic GSL biosynthetic genes by maintaining the DNA binding composition in the form of an SDI1-MYB28 complex, leading to down-regulation of GSL biosynthesis and prioritization of sulfate usage for primary metabolites under sulfur-deprived conditions.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Aarabi, FayezehUNSPECIFIEDorcid.org/0000-0002-5099-5400UNSPECIFIED
Kusajima, MiyukiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tohge, TakayukiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Konishi, TomokazuUNSPECIFIEDorcid.org/0000-0003-4826-6265UNSPECIFIED
Gigolashvili, TamaraUNSPECIFIEDorcid.org/0000-0002-0416-4796UNSPECIFIED
Takamune, MakikoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sasazaki, YokoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Watanabe, MutsumiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nakashita, HideoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fernie, Alisdair R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saito, KazukiUNSPECIFIEDorcid.org/0000-0001-6310-5342UNSPECIFIED
Takahashi, HidekiUNSPECIFIEDorcid.org/0000-0001-8439-0533UNSPECIFIED
Hubberten, Hans-MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoefgen, RainerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maruyama-Nakashita, AkikoUNSPECIFIEDorcid.org/0000-0002-3240-2843UNSPECIFIED
URN: urn:nbn:de:hbz:38-261859
DOI: 10.1126/sciadv.1601087
Journal or Publication Title: Sci. Adv.
Volume: 2
Number: 10
Date: 2016
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 2375-2548
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BHLH TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; SYSTEMS BIOLOGY; GENE-EXPRESSION; SULFATE UPTAKE; REGULATOR; MYB; METABOLOMICS; TRANSPORTERS; ASSIMILATIONMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/26185

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